代谢工程
格式化
代谢途径
背景(考古学)
DNA连接酶
生物合成
合成生物学
生物化学
生化工程
计算生物学
蛋白质工程
化学
计算机科学
酶
生物
工程类
古生物学
催化作用
作者
Jie Ren,Wei Wang,Jinglei Nie,Wenqiao Yuan,An‐Ping Zeng
出处
期刊:Advances in Biochemical Engineering / Biotechnology
日期:2022-01-01
卷期号:: 273-298
被引量:6
摘要
The glycine cleavage system (GCS) is a fundamental component of life, widely existing in microbes, plants, animals, and humans. A better understanding of the functionality and working mechanisms, and the engineering of the GCS have both scientific and practical impacts, which may lead to new knowledge and findings in life sciences, improved biomass production and human/animal health, efficient biosynthesis of chemicals, effective carbon fixation and global climate change mitigation. In this chapter, the GCS is first discussed in the context of the reductive glycine pathway (rGlyP), a recently proposed and appealing assimilation pathway of CO2 and formate, and its implementation and optimization in microorganisms for formatotrophic growth. Then, the present knowledge about the components, reactions, and working mechanisms of the GCS and related enzymes is reviewed. Particular emphasis is also placed on the conformational and structural features of the GCS proteins, especially the different forms of lipoylated H protein and its lipoylation by lipoate protein ligase (LplA). Subsequently, existing analytic methods for the components and reactions of the GCS and recent advances in quantitatively understanding and purposefully engineering the GCS are presented. Finally, perspectives of current state of the art in the GCS research are given and future research needs are highlighted.
科研通智能强力驱动
Strongly Powered by AbleSci AI