作者
Beate Rieblinger,Hicham Sid,Denise Duda,Tarik Bozoglu,Romina Klinger,Antonina Schlickenrieder,Kamila Lengyel,Krzysztof Flisikowski,Tatiana Flisikowska,Nina Simm,Alessandro Grodziecki,Carolin Perleberg,Andrea Bähr,Lucie Carrier,Mayuko Kurome,Valeri Zakhartchenko,Barbara Keßler,Eckhard Wolf,Lutz Kettler,Harald Luksch,Ibrahim T. Hagag,Daniel T. Wise,Jim Kaufman,Benedikt B. Kaufer,Christian Kupatt,Angelika Schnieke,Benjamin Schusser
摘要
Significance Genetically modified animals are crucial for functional studies and translational biomedical research. However, the generation of genome-edited large animals is time consuming and inefficient. Here, we report the generation of transgenic pigs and chickens that ubiquitously express Cas9 nuclease. The functionality of Cas9 was demonstrated for different target genes, a variety of cell types, and in vivo for the heart and the developing brain. Genome editing can now easily be carried out in vivo in specific organs or tissues without the need to generate germline-modified animals. These Cas9 transgenic animals provide an innovative and efficient resource for in vivo genome editing in biomedical and agricultural sciences.