结晶
蛋白质结晶
自愈水凝胶
成核
透析
透析管
蒸发
化学工程
图层(电子)
材料科学
膜
化学
高蛋白
膜蛋白
生物物理学
色谱法
纳米技术
生物化学
高分子化学
生物
有机化学
内科学
工程类
物理
热力学
医学
食品科学
作者
Shigeru Sugiyama,Keisuke Kakinouchi,Hiroaki Adachi,Tsugio Nagasawa,Noriaki Kunimune,Michio Murata,Yusuke Mori
标识
DOI:10.35848/1347-4065/abd0c7
摘要
Abstract This study focused on protein crystallization in high-strength hydrogels to expand the application of protein crystals. The results of our previous studies demonstrated several desirable merits, such as growth with a high nucleation rate and a high tolerance to solvent evaporation. However, the crystallization method faces the problem of handing protein solutions at high temperatures, which often causes the proteins to become inactivated or aggregated. This study established a new crystallization method that prevents high-temperature damage to proteins. This method offers a technique to osmose the protein from the top of a hydrogel layer and recover its crystals as the precipitant on the bottom of the hydrogel layer by using a handmade plate with a dialysis membrane. This study concentrated on the protein crystallization in hydrogels, but the results indicate that this method will be applicable to various proteins because it can always be operated at a low temperature.
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