自愈水凝胶
体内
细胞毒性
阿霉素
盐酸阿霉素
肽
化学
体外
药物输送
生物物理学
谷胱甘肽
氧化还原
材料科学
生物化学
高分子化学
有机化学
化疗
酶
外科
生物技术
生物
医学
作者
Yiping Li,Ying Zhu,Shiyao Luo,Yue He,Zhewei Huang,Juan Shen,Xiaoli Yuan,Zhuojian Lu,Han Han,Liang Ge,Lei Pan
标识
DOI:10.1166/jbn.2020.2974
摘要
In this study, we report a new ultrashort peptide (LOC), which forms a redox-sensitive hydrogel after cross-linking with the mild oxidant H 2 O 2 and used it for tumor-targeted delivery of doxorubicin hydrochloride (DOX). LOC gelled within a few minutes in low-concentration H 2 O 2 solution. The concentration of H 2 O 2 significantly altered the gelation time and mechanical properties of the hydrogel. The in vitro micromorphology, secondary structure and rheology characterization of cross-linked hydrogels confirmed the sensitivity and injectability to reducing agent. The cross-linked hydrogel had a strong drug loading capacity, and the drug was released in a GSH concentration-dependent manner, following the Fick diffusion model. In addition, the cross-linked hydrogel showed no cytotoxicity to normal fibroblasts, and no damage to the subcutaneous tissue of mice was observed. In vitro cytotoxicity experiments showed that the DOX-hydrogel system exhibited good anti-cancer efficacy. In vivo studies using 4T1 tumor-bearing mice showed that the DOX-hydrogel system had a significant inhibitory effect on tumors. Therefore, the newly designed redox-sensitive hydrogel can effectively enhance the therapeutic efficacy of DOX and reduce toxicity, making it an attractive biological material.
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