Design of Up-Conversion Fluorescence Imaging Device Based on Inverted Fluorescence Microscope

Fluorescence imaging is a widely used technique for microscopic observation in basic biomedical research. Currently, most of the commercial fluorescence microscopes are based on the design of down-conversion fluorescence reagent. In this paper, an up-conversion fluorescence imaging device based on inverted fluorescence microscope is designed. The device uses 980nm near-infrared as the excitation source and the inverted fluorescence microscope as the platform to transform its internal optical system and apply it to up-conversion fluorescence imaging. Through a large number of experimental studies, the stability and linearity of the equipment were verified, and the relationship between incubation time and intake of Hela cells was explored. The experimental results showed that when the laser power was 1. 5W and UCNPs@ DSPE-PE concentration was 0. 05mg/ml, the uptake of Hela cells reached the saturation state.