生化工程
环境友好型
化学
有机合成
过氧化氢
有机分子
基质(水族馆)
定向进化
范围(计算机科学)
生物催化
纳米技术
催化作用
组合化学
分子
有机化学
计算机科学
材料科学
生物化学
生物
反应机理
工程类
生态学
突变体
基因
程序设计语言
作者
Marie‐Cathérine Sigmund,Gerrit J. Poelarends
出处
期刊:Nature Catalysis
[Springer Nature]
日期:2020-09-16
卷期号:3 (9): 690-702
被引量:77
标识
DOI:10.1038/s41929-020-00507-8
摘要
Peroxygenases are attractive biocatalysts for the selective introduction of oxygen into organic molecules under mild conditions with hydrogen peroxide as the oxygen source. In addition to the identification of primary peroxygenases, different classes of enzymes were shown to display promiscuous peroxygenase activity. Even though enzymes with peroxygenase activity are promising industrial biocatalysts, further optimization of their properties is required for their effective use in industrial applications. Here we give a comprehensive overview of enzymes with peroxygenase activity and review diverse strategies, including directed evolution, rational approaches and the assistance of small functional molecules to improve the expression, catalytic activity, substrate scope or selectivity of these promising enzymes. Furthermore, we discuss the exploration of modified or unnatural cofactors to design artificial peroxygenases for desired reactions. The rapidly expanding field of hydrogen peroxide-utilizing enzymes bears a great potential to provide biocatalysts for selective oxyfunctionalization chemistry, contributing to the development of environmentally friendly and sustainable oxidation processes. Peroxygenases are promising enzymes to contribute to more environmentally friendly and sustainable oxidation reactions in industry. This Review Article gives a comprehensive overview about enzymes with peroxygenase activity and strategies to engineer them for desired reactions and enhanced performances.
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