亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Characterization of Polysorbate Ester Fractions and Implications in Protein Drug Product Stability

聚山梨酯 化学 胶束 肺表面活性物质 色谱法 生物制药 临界胶束浓度 水解 溶解度 有机化学 化学工程 生物化学 水溶液 遗传学 生物 工程类
作者
Anthony A. G. Tomlinson,Isidro E. Zarraga,Barthélemy Demeule
出处
期刊:Molecular Pharmaceutics [American Chemical Society]
卷期号:17 (7): 2345-2353 被引量:42
标识
DOI:10.1021/acs.molpharmaceut.0c00093
摘要

Polysorbates (PS) are commonly used surfactants in biopharmaceutical protein formulations. However, they are susceptible to a variety of degradation pathways, including chemical hydrolysis, oxidation, and enzymatic hydrolysis. Polysorbates are also heterogeneous mixtures, and it has been observed that the patterns of degradation can be strikingly different between the different pathways. Polysorbates (PS20 and PS80) were fractionated, and the fractions were characterized for their physicochemical properties, such as surface tension, micelle size, critical micelle concentration (CMC), and agitation protection for a monoclonal antibody (mAb). This report seeks to use this information to inform how these properties might change in polysorbates as they degrade in biopharmaceutical formulations. The physicochemical properties examined shed light on some of the differences between PS types and the different chemical components of polysorbates. Differences in physicochemical properties for fractionated polysorbates could help inform biopharmaceutical formulations that use PS surfactants. Importantly, they show that subspecies of PS20 are far more distinct from each other than those of PS80. Fractions of PS20 showed highly different critical micelle concentrations and effects on equilibrium surface tension. These differences, and possibly other untested parameters, led to vastly different protective effects for a model mAb under agitation stress. Additionally, the propensity of various PS fractions to form micelles can impact both polysorbate quantitation measurements, some of which rely on micellization, and the effective solubility of hydrophobic compounds (e.g., fatty acids) in the surfactant solution.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
直率的笑翠完成签到 ,获得积分10
7秒前
hgs完成签到,获得积分10
12秒前
12秒前
MchemG应助科研通管家采纳,获得10
33秒前
JamesPei应助科研通管家采纳,获得10
33秒前
隐形曼青应助科研通管家采纳,获得10
34秒前
Kevin完成签到,获得积分10
1分钟前
1分钟前
辉哥发布了新的文献求助10
1分钟前
1分钟前
1分钟前
董可以发布了新的文献求助10
1分钟前
英俊的铭应助董可以采纳,获得10
1分钟前
curtain完成签到,获得积分10
2分钟前
大个应助科研通管家采纳,获得10
2分钟前
MchemG应助科研通管家采纳,获得10
2分钟前
所所应助科研通管家采纳,获得10
2分钟前
科研通AI2S应助科研通管家采纳,获得10
2分钟前
2分钟前
落寞书易完成签到 ,获得积分10
2分钟前
2分钟前
现实的小霸王完成签到,获得积分10
2分钟前
3分钟前
Xw完成签到,获得积分10
3分钟前
科研通AI5应助迷人问兰采纳,获得10
3分钟前
Hello应助LSH970829采纳,获得10
3分钟前
Xw发布了新的文献求助10
3分钟前
寒冷的应助核桃采纳,获得30
4分钟前
wen发布了新的文献求助10
4分钟前
隐形曼青应助科研通管家采纳,获得10
4分钟前
科研通AI2S应助科研通管家采纳,获得10
4分钟前
MchemG应助科研通管家采纳,获得10
4分钟前
wen完成签到,获得积分10
4分钟前
4分钟前
4分钟前
4分钟前
yar应助wen采纳,获得10
4分钟前
核桃发布了新的文献求助30
4分钟前
迷人问兰发布了新的文献求助10
4分钟前
4分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990084
求助须知:如何正确求助?哪些是违规求助? 3532108
关于积分的说明 11256447
捐赠科研通 3271016
什么是DOI,文献DOI怎么找? 1805171
邀请新用户注册赠送积分活动 882270
科研通“疑难数据库(出版商)”最低求助积分说明 809228