开始成熟
浆果
转录组
葡萄
鲜食葡萄
生物
RNA序列
基因
植物
基因表达
园艺
遗传学
作者
Li L,Sun LingJun,Yinshan Guo,Hong Lin,Zhendong Liu,Kun Li,Xiuwu Guo
出处
期刊:PLOS ONE
[Public Library of Science]
日期:2020-08-17
卷期号:15 (8): e0237526-e0237526
被引量:21
标识
DOI:10.1371/journal.pone.0237526
摘要
Berry firmness is one of the main selection criteria for table grape breeding. However, the underlying genetic determinants and mechanisms involved in gene expression during berry development are still poorly understood. In this study, eighteen libraries sampled from Vitis vinifera L. cv. ‘Red Globe’ and ‘Muscat Hamburg’ at three developmental stages (preveraison, veraison and maturation) were analyzed by RNA sequencing (RNA-Seq). The firmness of ‘Red Globe’ was significantly higher than that of ‘Muscat Hamburg’ at the three developmental stages. In total, a set of 4,559 differentially expressed genes (DEGs) was identified between ‘Red Globe’ and ‘Muscat Hamburg’ in the preveraison (2,259), veraison (2030) and maturation stages (2682), including 302 transcription factors (TFs). Weighted gene coexpression network analysis (WGCNA) showed that 23 TFs were predicted to be highly correlated with fruit firmness and propectin content. In addition, the differential expression of the PE, PL, PG, β-GAL, GATL, WAK, XTH and EXP genes might be the reason for the differences in firmness between ‘Red Globe’ and ‘Muscat Hamburg’. The results will provide new information for analysis of grape berry firmness and softening.
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