毕赤酵母
信号肽
内生
分泌物
异源的
分泌蛋白
毕赤酵母
分泌途径
重组DNA
生物化学
酿酒酵母
绿色荧光蛋白
生物
细胞生物学
酵母
内质网
基因
高尔基体
作者
Shuli Liang,Cheng Li,Yanrui Ye,Ying Lin
标识
DOI:10.1007/s10529-012-1055-8
摘要
By predicting the potential signal peptides from proteins that are naturally secreted by Pichia pastoris, we identified three possible endogenous signal peptides: Scw, Dse and Exg. We compared their capability to mediate the secretion of enhanced green fluorescent protein (EGFP) and Candida antarctica lipase B (CALB) with that of the Saccharomyces cerevisiae α-factor prepro-signal. EGFP entered the secretory pathway of P. pastoris and was efficiently secreted into the culture medium by all three endogenous peptides. Further, these three putative endogenous signal peptides were also effective in secreting CALB. These endogenous signal peptides thus have the potential to mediate the efficient secretion of heterologous proteins in P. pastoris.
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