化学
色谱法
亲水作用色谱法
洗脱
静电学
相(物质)
分辨率(逻辑)
离子交换
核苷酸
离子色谱法
静电相互作用
氨基酸
反相色谱法
离子
高效液相色谱法
有机化学
化学物理
生物化学
物理化学
人工智能
基因
计算机科学
摘要
If an ion-exchange column is eluted with a predominantly organic mobile phase, then solutes can be retained through hydrophilic interaction even if they have the same charge as the stationary phase. This combination is termed electrostatic repulsion-hydrophilic interaction chromatography (ERLIC). With mixtures of solutes that differ greatly in charge, repulsion effects can be exploited to selectively antagonize the retention of the solutes that normally would be the best retained. This permits the isocratic resolution of mixtures that normally require gradients, including peptides, amino acids, and nucleotides. ERLIC affords convenient separations of highly charged peptides that cannot readily be resolved by other means. In addition, phosphopeptides can be isolated selectively from a tryptic digest.
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