vsiRNAs derived from the miRNA-generating sites of pri-tae-miR159a based on the BSMV system play positive roles in the wheat response to Puccinia striiformis f. sp. tritici through the regulation of taMyb3 expression

生物 基因沉默 小RNA 基因 掷骰子 遗传学 表型 RNA干扰 核糖核酸
作者
Hao Feng,Qiong Zhang,Huayi Li,Xiaojie Wang,Xiaoyuan Duan,Bing Wang,Zhensheng Kang
出处
期刊:Plant Physiology and Biochemistry [Elsevier BV]
卷期号:68: 90-95 被引量:20
标识
DOI:10.1016/j.plaphy.2013.04.008
摘要

Plants live in a complex environment, exposed to stresses, such as unsuitable climates, pests and pathogenic microorganisms. Pathogens are one of the most serious factors that threaten plant growth. Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases worldwide. Virus-induced gene silencing (VIGS) is a popular tool for the functional analysis of wheat genes, generating abundant small RNAs (sRNAs). sRNAs are key components in gene regulatory networks, silencing corresponding genes at the post-transcriptional level. In this study, we transduced pri-tae-miR159a into plant tissues using the barley stripe mosaic virus (BSMV) system, and demonstrated that vsiRNAs were generated from the same miRNAs generating sites of pri-tae-miR159a, with the function of Dicer RNase III-like classes of endonucleases (DCL4). In addition, the accumulation of vsiRNAs in wheat leaves challenged with Pst Chinese yellow rust 23 (CYR23), resulted in a resistant phenotype, and in the compatible interaction, the sporation of Pst was limited. Whereas, infection with a control construct had no effect on the resistance or susceptibility. The results of the histological observation also supported these phenotype changes. Interestingly, vsiRNAs were also involved in the interactions between wheat and Pst through the tae-miR159-mediated regulation of taMyb3 expression. Moreover, these results also supported the speculation that vsiRNAs were generated from the same sites of pri-tae-miR159a. These studies indicated that vsiRNAs from miRNAs generating sites of pri-tae-miR159a based on the BSMV system play positive roles in the wheat response to Pst through the regulation of taMyb3 expression.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
leei完成签到,获得积分10
2秒前
meng123发布了新的文献求助10
2秒前
小马甲应助c123采纳,获得10
3秒前
莎莎士比亚完成签到,获得积分10
5秒前
LOST完成签到 ,获得积分10
5秒前
6秒前
袁凯文发布了新的文献求助10
6秒前
共享精神应助老菜鸟321采纳,获得10
6秒前
WUWEI发布了新的文献求助10
7秒前
xiaoW完成签到,获得积分10
7秒前
9秒前
牛太虚完成签到,获得积分10
9秒前
SciGPT应助科多兽骑士采纳,获得10
9秒前
11秒前
gjm完成签到,获得积分10
11秒前
SciGPT应助zj采纳,获得10
12秒前
Ava应助阿湫采纳,获得10
12秒前
meng123完成签到,获得积分20
13秒前
x5kyi完成签到,获得积分10
14秒前
爆米花应助肖遥采纳,获得10
15秒前
Xx完成签到,获得积分10
15秒前
15秒前
18秒前
烟里戏完成签到 ,获得积分10
20秒前
shuangfeng1853完成签到 ,获得积分10
20秒前
林子青发布了新的文献求助10
20秒前
21秒前
aa完成签到,获得积分10
21秒前
CXC完成签到,获得积分10
21秒前
23秒前
Zzz发布了新的文献求助10
23秒前
上官若男应助袁凯文采纳,获得10
24秒前
24秒前
褚晣完成签到,获得积分10
24秒前
ATTENTION完成签到,获得积分10
25秒前
25秒前
周欣玙完成签到,获得积分10
25秒前
25秒前
学不懂数学应助as采纳,获得30
25秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
Research on Disturbance Rejection Control Algorithm for Aerial Operation Robots 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038524
求助须知:如何正确求助?哪些是违规求助? 3576221
关于积分的说明 11374737
捐赠科研通 3305912
什么是DOI,文献DOI怎么找? 1819354
邀请新用户注册赠送积分活动 892688
科研通“疑难数据库(出版商)”最低求助积分说明 815048