清晨好,您是今天最早来到科研通的研友!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您科研之路漫漫前行!

vsiRNAs derived from the miRNA-generating sites of pri-tae-miR159a based on the BSMV system play positive roles in the wheat response to Puccinia striiformis f. sp. tritici through the regulation of taMyb3 expression

生物 基因沉默 小RNA 基因 掷骰子 遗传学 表型 RNA干扰 核糖核酸
作者
Hao Feng,Qiong Zhang,Huayi Li,Xiaojie Wang,Xiaoyuan Duan,Bing Wang,Zhensheng Kang
出处
期刊:Plant Physiology and Biochemistry [Elsevier BV]
卷期号:68: 90-95 被引量:20
标识
DOI:10.1016/j.plaphy.2013.04.008
摘要

Plants live in a complex environment, exposed to stresses, such as unsuitable climates, pests and pathogenic microorganisms. Pathogens are one of the most serious factors that threaten plant growth. Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases worldwide. Virus-induced gene silencing (VIGS) is a popular tool for the functional analysis of wheat genes, generating abundant small RNAs (sRNAs). sRNAs are key components in gene regulatory networks, silencing corresponding genes at the post-transcriptional level. In this study, we transduced pri-tae-miR159a into plant tissues using the barley stripe mosaic virus (BSMV) system, and demonstrated that vsiRNAs were generated from the same miRNAs generating sites of pri-tae-miR159a, with the function of Dicer RNase III-like classes of endonucleases (DCL4). In addition, the accumulation of vsiRNAs in wheat leaves challenged with Pst Chinese yellow rust 23 (CYR23), resulted in a resistant phenotype, and in the compatible interaction, the sporation of Pst was limited. Whereas, infection with a control construct had no effect on the resistance or susceptibility. The results of the histological observation also supported these phenotype changes. Interestingly, vsiRNAs were also involved in the interactions between wheat and Pst through the tae-miR159-mediated regulation of taMyb3 expression. Moreover, these results also supported the speculation that vsiRNAs were generated from the same sites of pri-tae-miR159a. These studies indicated that vsiRNAs from miRNAs generating sites of pri-tae-miR159a based on the BSMV system play positive roles in the wheat response to Pst through the regulation of taMyb3 expression.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
缥缈的闭月完成签到,获得积分10
17秒前
六一完成签到 ,获得积分10
25秒前
怡然的煜城完成签到 ,获得积分10
27秒前
40秒前
玄之又玄完成签到,获得积分10
45秒前
Evangeline993完成签到 ,获得积分10
50秒前
可飞完成签到,获得积分10
1分钟前
绿色心情完成签到 ,获得积分10
1分钟前
飞翔的企鹅完成签到,获得积分10
1分钟前
wBw完成签到,获得积分10
1分钟前
zhangsan完成签到,获得积分10
1分钟前
1分钟前
TheaGao完成签到 ,获得积分10
1分钟前
高海龙完成签到 ,获得积分10
2分钟前
aldehyde应助哥哥采纳,获得10
2分钟前
程小柒完成签到 ,获得积分10
2分钟前
复杂的听白给复杂的听白的求助进行了留言
2分钟前
如意如意按我心意完成签到 ,获得积分10
2分钟前
碗碗豆喵完成签到 ,获得积分10
2分钟前
科研通AI2S应助科研通管家采纳,获得10
2分钟前
科研通AI2S应助科研通管家采纳,获得10
2分钟前
alanbike完成签到,获得积分10
2分钟前
Santiago完成签到,获得积分10
2分钟前
哥哥完成签到,获得积分10
2分钟前
huangzsdy完成签到,获得积分10
2分钟前
LeoBigman完成签到 ,获得积分10
2分钟前
zzgpku完成签到,获得积分0
3分钟前
Tong完成签到,获得积分0
3分钟前
3分钟前
阿柴_Htao完成签到 ,获得积分10
4分钟前
拉长的秋白完成签到 ,获得积分10
4分钟前
标致的泥猴桃完成签到,获得积分10
4分钟前
月儿完成签到 ,获得积分10
4分钟前
elisa828发布了新的文献求助20
4分钟前
nini完成签到,获得积分10
4分钟前
4分钟前
流氓恐龙完成签到,获得积分10
4分钟前
爆米花应助常有李采纳,获得10
4分钟前
满意的伊完成签到,获得积分10
4分钟前
刘丰完成签到 ,获得积分10
4分钟前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
Immigrant Incorporation in East Asian Democracies 600
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3968521
求助须知:如何正确求助?哪些是违规求助? 3513358
关于积分的说明 11167301
捐赠科研通 3248700
什么是DOI,文献DOI怎么找? 1794453
邀请新用户注册赠送积分活动 875030
科研通“疑难数据库(出版商)”最低求助积分说明 804664