Development, Optimization and in vitro-in vivo Evaluation of Pioglitazone- Loaded Jackfruit Seed Starch-Alginate Beads

The present investigation describes development and optimization of pioglitazone-loaded jackfruit seed starch (JFSS)-alginate beads by ionotropic-gelation using 32 factorial design. The effect of polymer-blend ratio and CaCl2 concentration on the drug encapsulation efficiency (DEE, %), and cumulative drug release after 10 hours (R10h, %) was optimized. The DEE (%) of these beads were 64.80 ± 1.92 to 94.07 ± 3.82 % with sustained in vitro drug release of 64.±1.83 to 92.66 ± 4.54 % over 10 hours. The in vitro drug release from these beads followed controlled-release pattern with super case-II transport. Particle size range of these beads was 0.77 ± 0.04 to 1.24 ± 0.09 mm. The beads were also characterized by SEM and FTIR. The swelling of these beads was influenced by pH of the test medium. The optimized pioglitazone-loaded JFSS-alginate beads showed significant hypoglycemic effect in alloxan-induced diabetic rats over prolonged period after oral administration. Keywords: Jackfruit seed starch, Alginate, Drug release, Controlled delivery, Pioglitazone, Factorial design.