Compact Vesicles Self-Assembled from Binary Graft Copolymers with High Hydrophilic Fraction for Potential Drug/Protein Delivery

Hollow vesicles self-assembled from amphiphilic copolymers are of great interest in biomedicine field as drug and protein carriers. Efficient preparation of polymeric vesicles with high stability in vivo is highly desirable. Herein, a novel cooperative self-assembly of two graft copolymers (GCPs) with reversed hydrophilic-hydrophobic segments is investigated to achieve morphology control for biomedical application. Interestingly, nanosized vesicles are obtained for the binary system with relatively high hydrophilic fraction (fhydrophilic, ∼60%), contrary to what is found in its single-component counterpart. The cooperative self-assembly endowed the hybrid vesicles with excellent resistance to protein adsorption, prolonged blood circulation time, as well as low leakage of hydrophilic drugs/proteins. Furthermore, the biological activity of the protein is well preserved inside the cooperative vesicles, making it a promising candidate as the protein carrier.