氯
化学
整合子
磺胺甲恶唑
细菌
核化学
假单胞菌
微生物学
色谱法
抗生素
抗生素耐药性
生物化学
生物
有机化学
遗传学
作者
Tianyang Zhang,Yaru Hu,Lei Jiang,Shijie Yao,Kuangfei Lin,Yanbo Zhou,Changzheng Cui
标识
DOI:10.1016/j.cej.2018.09.218
摘要
This study investigated the reduction of one antibiotic resistance gene (ARG, sul1) and one integron (intI1) within a multiple-antibiotic-resistant bacterium (ARB), Pseudomonas. HLS-6, during UV, chlorination and UV/chlorination. This bacterial strain was easily inactivated by these three methods, but its gene inactivation was not so easy. Two short target gene sequences (sul1-qPCR and intI1-qPCR) were selected for quantitative analysis, and another two longer ones (sul1-PCR and intI1-PCR) were used for qualitative identification. During the initial reaction time (<20 min), the degradation rate order of sul1-qPCR and intI1-qPCR was as follows: UV/chlorination>chlorination>UV. The log reduction of sul1-qPCR and intI1-qPCR in UV/chlorination achieved >3.50 and 4.00 log, respectively. The removal efficiency of sul1-PCR and intI1-PCR was also identified by gel electrophoresis analysis, which further confirmed the advantage of UV/chlorination treatment on DNA damage. Among the radicals produced in UV/chlorination, only the reactive chlorine species (Cl⋅, Cl2⋅− and ClO⋅) can degrade the target genes. Under the condition of low chlorine dosage, sul1-qPCR was easier to be removed than intI1-qPCR by UV/chlorination. The log reduction of both sul1-qPCR and intI1-qPCR decreased with pH increasing during UV/chlorination. In the presence of sulfamethoxazole, UV/chlorination showed more advantages than direct UV and chlorination in the simultaneous removal of antibiotics, ARBs and ARGs.
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