Extraction, identification and structure-activity relationship of antioxidant peptides from sesame (Sesamum indicum L.) protein hydrolysate

化学 水解物 抗氧化剂 阿布茨 DPPH 芝麻 色谱法 电喷雾电离 串联质谱法 生物化学 水解 质谱法 生物 园艺
作者
Xin Lu,Lixia Zhang,Qiang Sun,Guohui Song,Jinian Huang
出处
期刊:Food Research International [Elsevier]
卷期号:116: 707-716 被引量:101
标识
DOI:10.1016/j.foodres.2018.09.001
摘要

To elucidate the sequence, origin and structure-activity relationship of antioxidant peptides from sesame protein, sesame protein was hydrolysed by a dual-enzyme system comprised alcalase and trypsin, then this hydrolysate was fractionated by ultrafiltration and preparative HPLC. Subsequently, peptides in the high antioxidant activity fraction were identified by nano liquid chromatography-electrospray ionization-tandem mass spectrometry (Nano-LC-ESI-MS/MS), finally the structure-activity relationship of antioxidant peptide with the strongest activity in the sesame peptides was illustrated by comparative molecular field analysis (CoMFA). The results showed that seven novel antioxidant peptides were discovered, their sequences were as follows, RDRHQKIG, TDRHQKLR, MNDRVNQGE, RENIDKPSRA, SYPTECRMR, GGVPRSGEQEQQ and AGEQGFEYVTFR. The SYPTECRMR was the hydrolysate of 2S albumin, the others derived from 11S globulin. The SYPTECRMR whose IC50 Values of DPPH and ABTS were 0.105 mg/mL and 0.004 mg/mL respectively exhibited the highest antioxidant activity among the seven sesame peptides. The active site of SYPTECRMR tended to locate on Cys6 and Met8. A positive correlation between Cys6, Met8, the bulky C-terminal amino acid residue (Arg9), the negative charged group around sulphur-containing amino acids and the antioxidant activity of SYPTECRMR was observed from the CoMFA model. The results presented herein suggested that sesame protein hydrolysates have potential applications in functional food due to their high antioxidant activity, the CoMFA model could provide insight into the structure-activity relationship of antioxidant peptide, which is useful to screen, identify and design the novel antioxidant peptide.
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