谷胱甘肽
谷胱甘肽二硫化物
氧化应激
程序性细胞死亡
甘氨酸
生物化学
丝氨酸
抗氧化剂
生物
微生物学
细菌
先天免疫系统
化学
氨基酸
酶
细胞凋亡
遗传学
受体
作者
Tian-shun Kou,Jiahan Wu,Xuan-wei Chen,Zhuang‐Gui Chen,Jun Zheng,Bo Peng
出处
期刊:Redox biology
[Elsevier]
日期:2022-10-21
卷期号:58: 102512-102512
被引量:30
标识
DOI:10.1016/j.redox.2022.102512
摘要
Pathogenic strains of bacteria are often highly adept at evading serum-induced cell death, which is an essential complement-mediated component of the innate immune response. This phenomenon, known as serum-resistance, is poorly understood, and as a result, no effective clinical tools are available to restore serum-sensitivity to pathogenic bacteria. Here, we provide evidence that exogenous glycine reverses defects in glycine, serine and threonine metabolism associated with serum resistance, restores susceptibility to serum-induced cell death, and alters redox balance and glutathione (GSH) metabolism. More specifically, in Vibrio alginolyticus and Escherichia coli, exogenous glycine promotes oxidation of GSH to GSH disulfide (GSSG), disrupts redox balance, increases oxidative stress and reduces membrane integrity, leading to increased binding of complement. Antioxidant or ROS scavenging agents abrogate this effect and agents that generate or potentiate oxidation stimulate serum-mediated cell death. Analysis of several clinical isolates of E. coli demonstrates that glutathione metabolism is repressed in serum-resistant bacteria. These data suggest a novel mechanism underlying serum-resistance in pathogenic bacteria, characterized by an induced shift in the GSH/GSSG ratio impacting redox balance. The results could potentially lead to novel approaches to manage infections caused by serum-resistant bacteria both in aquaculture and human health.
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