The OPEN STOMATA1–SPIRAL1 module regulates microtubule stability during abscisic acid-induced stomatal closure in Arabidopsis

脱落酸 微管 生物 拟南芥 细胞生物学 结束语(心理学) 突变体 植物 生物化学 基因 经济 市场经济
作者
Pan Wang,Sijia Qi,Xiaohong Wang,Liru Dou,Mengao Jia,Tonglin Mao,Yushuang Guo,Xiangfeng Wang
出处
期刊:The Plant Cell [Oxford University Press]
卷期号:35 (1): 260-278 被引量:32
标识
DOI:10.1093/plcell/koac307
摘要

Abstract Drought stress triggers abscisic acid (ABA) signaling in guard cells and induces stomatal closure to prevent water loss in land plants. Stomatal movement is accompanied by reorganization of the cytoskeleton. Cortical microtubules disassemble in response to ABA, which is required for stomatal closure. However, how ABA signaling regulates microtubule disassembly is unclear, and the microtubule-associated proteins (MAPs) involved in this process remain to be identified. In this study, we show that OPEN STOMATA 1 (OST1), a central component in ABA signaling, mediates microtubule disassembly during ABA-induced stomatal closure in Arabidopsis thaliana. We identified the MAP SPIRAL1 (SPR1) as the substrate of OST1. OST1 interacts with and phosphorylates SPR1 at Ser6, which promotes the disassociation of SPR1 from microtubules and facilitates microtubule disassembly. Compared with the wild type, the spr1 mutant exhibited significantly greater water loss and reduced ABA responses, including stomatal closure and microtubule disassembly in guard cells. These phenotypes were restored by introducing the phosphorylated active form of SPR1. Our findings demonstrate that SPR1 positively regulates microtubule disassembly during ABA-induced stomatal closure, which depends on OST1-mediated phosphorylation. These findings reveal a specific connection between a core component of ABA signaling and MAPs.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Yt完成签到 ,获得积分10
刚刚
怕孤独的访云完成签到 ,获得积分10
刚刚
噗宝凹发布了新的文献求助10
刚刚
赘婿应助xiaofei666采纳,获得10
1秒前
1秒前
2秒前
科目三应助Ampere采纳,获得10
3秒前
3秒前
蓝胖子发布了新的文献求助10
3秒前
背后孤晴发布了新的文献求助10
4秒前
脑洞疼应助coldstork采纳,获得10
4秒前
4秒前
5秒前
5秒前
zs发布了新的文献求助10
5秒前
leo发布了新的文献求助10
6秒前
8秒前
8秒前
9秒前
文艺的土豆完成签到,获得积分10
10秒前
蓝胖子发布了新的文献求助10
10秒前
少年啊完成签到,获得积分10
11秒前
11秒前
紫色翡翠完成签到,获得积分10
11秒前
敏敏发布了新的文献求助10
12秒前
卡皮巴拉发布了新的文献求助10
12秒前
13秒前
13秒前
X519664508完成签到,获得积分0
14秒前
14秒前
15秒前
15秒前
coldstork发布了新的文献求助10
16秒前
zs发布了新的文献求助10
16秒前
diaiyi完成签到 ,获得积分10
16秒前
蓝胖子发布了新的文献求助10
17秒前
打打应助背后孤晴采纳,获得10
17秒前
yikeguozi完成签到,获得积分10
17秒前
Ampere发布了新的文献求助10
17秒前
大个应助科研通管家采纳,获得10
18秒前
高分求助中
歯科矯正学 第7版(或第5版) 1004
Smart but Scattered: The Revolutionary Executive Skills Approach to Helping Kids Reach Their Potential (第二版) 1000
Semiconductor Process Reliability in Practice 720
GROUP-THEORY AND POLARIZATION ALGEBRA 500
Mesopotamian divination texts : conversing with the gods : sources from the first millennium BCE 500
Days of Transition. The Parsi Death Rituals(2011) 500
The Heath Anthology of American Literature: Early Nineteenth Century 1800 - 1865 Vol. B 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3229292
求助须知:如何正确求助?哪些是违规求助? 2877020
关于积分的说明 8197467
捐赠科研通 2544342
什么是DOI,文献DOI怎么找? 1374310
科研通“疑难数据库(出版商)”最低求助积分说明 646923
邀请新用户注册赠送积分活动 621738