Cuproptosis-Related MiR-21-5p/FDX1 Axis in Clear Cell Renal Cell Carcinoma and Its Potential Impact on Tumor Microenvironment

肾透明细胞癌 癌症研究 肿瘤微环境 细胞生长 免疫系统 基因敲除 生物 细胞 细胞培养 肾细胞癌 医学 病理 免疫学 肿瘤细胞 遗传学
作者
Mingyue Xie,Bo Cheng,Shuang Yu,Yajie He,Yu Cao,Tiejun Zhou,Kun Han,Rongyang Dai,Ronghao Wang
出处
期刊:Cells [MDPI AG]
卷期号:12 (1): 173-173 被引量:31
标识
DOI:10.3390/cells12010173
摘要

As a newly identified type of programmed cell death, cuproptosis may have an impact on cancer development, including clear cell renal cell carcinoma (ccRCC). Herein, we first noticed that the expression levels of cuproptosis regulators exhibited a tight correlation with the clinicopathological characteristics of ccRCC. The cuproptosis-sensitive sub-type (CSS), classified via consensus clustering analysis, harbored a higher overall survival rate compared to the cuproptosis-resistant sub-type (CRS), which may have resulted from the differential infiltration of immune cells. FDX1, the cuproptosis master regulator, was experimentally determined as a tumor suppressor in ccRCC cells by suppressing the cell growth and cell invasion of ACHN and OSRC-2 cells in a cuproptosis-dependent and -independent manner. The results from IHC staining also demonstrated that FDX1 expression was negatively correlated with ccRCC tumor initiation and progression. Furthermore, we identified the miR-21-5p/FDX1 axis in ccRCC and experimentally verified that miR-21-5p directly binds the 3′-UTR of FDX1 to mediate its degradation. Consequently, a miR-21-5p inhibitor suppressed the cell growth and cell invasion of ACHN and OSRC-2 cells, which could be compensated by FDX1 knockdown, reinforcing the functional linkage between miR-21-5p and FDX1 in ccRCC. Finally, we evaluated the ccRCC tumor microenvironment under the miR-21-5p/FDX1 axis and noted that this axis was strongly associated with the infiltration of immune cells such as CD4+ T cells, Treg cells, and macrophages, suggesting that this signaling axis may alter microenvironmental components to drive ccRCC progression. Overall, this study constructed the miR-21-5p/FDX1 axis in ccRCC and analyzed its potential impact on the tumor microenvironment, providing valuable insights to improve current ccRCC management.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
在水一方应助活力初蝶采纳,获得10
1秒前
2秒前
2秒前
淡淡溪流完成签到,获得积分10
2秒前
Emma应助张三采纳,获得10
2秒前
3秒前
SciGPT应助ebbinghuazhu采纳,获得10
4秒前
ClaudiaCY发布了新的文献求助10
4秒前
4秒前
4秒前
yhc发布了新的文献求助10
5秒前
5秒前
5秒前
科研通AI2S应助xiong采纳,获得10
5秒前
JamesPei应助小前途采纳,获得10
5秒前
6秒前
lanwei完成签到,获得积分10
7秒前
7秒前
忘忧草发布了新的文献求助10
7秒前
科研通AI2S应助LARS采纳,获得10
7秒前
FIN发布了新的文献求助60
7秒前
Dr.Lyo完成签到,获得积分10
7秒前
8秒前
wqq发布了新的文献求助20
8秒前
魔幻灵槐发布了新的文献求助10
8秒前
风中的逍遥完成签到,获得积分10
8秒前
醒醒发布了新的文献求助10
9秒前
9秒前
神经哇发布了新的文献求助10
9秒前
活力初蝶完成签到,获得积分20
9秒前
9秒前
共享精神应助小布采纳,获得10
9秒前
10秒前
11秒前
11秒前
11秒前
light派完成签到,获得积分10
12秒前
科研通AI2S应助肥团采纳,获得10
12秒前
张润泽完成签到 ,获得积分10
13秒前
高分求助中
Lire en communiste 1000
Becoming: An Introduction to Jung's Concept of Individuation 600
中国氢能技术发展路线图研究 500
Communist propaganda: a fact book, 1957-1958 500
Briefe aus Shanghai 1946‒1952 (Dokumente eines Kulturschocks) 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
A new species of Velataspis (Hemiptera Coccoidea Diaspididae) from tea in Assam 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3168966
求助须知:如何正确求助?哪些是违规求助? 2820245
关于积分的说明 7929811
捐赠科研通 2480332
什么是DOI,文献DOI怎么找? 1321320
科研通“疑难数据库(出版商)”最低求助积分说明 633191
版权声明 602497