亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Using Passive Microrheology to Measure the Evolution of the Rheological Properties of NIST mAb Formulations during Adsorption to the Air–Water Interface

微流变学 NIST公司 度量(数据仓库) 流变学 吸附 化学 接口(物质) 材料科学 分析化学(期刊) 热力学 纳米技术 化学工程 化学物理 物理化学 色谱法 物理 计算机科学 工程类 数据挖掘 吉布斯等温线 自然语言处理
作者
Estephanie Laura Nottar Escobar,M. Coleman Vaclaw,Joseph T. Lozenski,Prajnaparamita Dhar
出处
期刊:Langmuir [American Chemical Society]
标识
DOI:10.1021/acs.langmuir.3c03658
摘要

The development of novel protein-based therapeutics, such as monoclonal antibodies (mAbs), is often limited due to challenges associated with maintaining the stability of these formulations during manufacturing, storage, and clinical administration. An undesirable consequence of the instability of protein therapeutics is the formation of protein particles. MAbs can adsorb to interfaces and have the potential to undergo partial unfolding as well as to form viscoelastic gels. Further, the viscoelastic properties may be correlated with their aggregation potential. In this work, a passive microrheology technique was used to correlate the evolution of surface adsorption with the evolution of surface rheology of the National Institute of Standards and Technology (NIST) mAb reference material (NIST mAb) and interface-induced subvisible protein particle formation. The evolution of the surface adsorption and interfacial shear rheological properties of the NIST mAb was recorded in four formulation conditions: two different buffers (histidine vs phosphate-buffered saline) and two different pHs (6.0 and 7.6). Our results together demonstrate the existence of multiple stages for both surface adsorption and surface rheology, characterized by an induction period that appears to be purely viscous, followed by a sharp increase in protein molecules at the interface when the film rheology is viscoelastic and ultimately a slowdown in the surface adsorption that corresponds to the formation of solid-like or glassy films at the interface. When the transitions between the different stages occurred, they were dependent on the buffer/pH of the formulations. The onset of these transitions can also be correlated to the number of protein particles formed at the interface. Finally, the addition of polysorbate 80, an FDA-approved surfactant used to mitigate protein particle formation, led to the interface being surfactant-dominated, and the resulting interface remained purely viscous.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
2秒前
体贴花卷发布了新的文献求助10
7秒前
physicalproblem完成签到,获得积分10
26秒前
40秒前
Ken完成签到,获得积分10
59秒前
1分钟前
风中凡霜完成签到,获得积分10
1分钟前
风中凡霜发布了新的文献求助10
1分钟前
1分钟前
大模型应助体贴花卷采纳,获得10
1分钟前
1分钟前
zxr发布了新的文献求助10
1分钟前
zhengzhster完成签到,获得积分10
1分钟前
2分钟前
小二郎应助幸福的小霜采纳,获得10
2分钟前
2分钟前
科研通AI2S应助科研通管家采纳,获得10
3分钟前
科研通AI2S应助科研通管家采纳,获得10
3分钟前
爆米花应助科研通管家采纳,获得10
3分钟前
两棵树完成签到,获得积分10
3分钟前
体贴花卷发布了新的文献求助10
3分钟前
3分钟前
拼搏的败完成签到 ,获得积分10
3分钟前
月亮完成签到 ,获得积分10
3分钟前
binyao2024完成签到,获得积分10
3分钟前
4分钟前
ptn__z发布了新的文献求助10
4分钟前
ptn__z完成签到,获得积分10
4分钟前
科研通AI2S应助科研通管家采纳,获得10
5分钟前
5分钟前
体贴花卷发布了新的文献求助10
5分钟前
情怀应助小鹿采纳,获得10
5分钟前
小鹿完成签到,获得积分10
5分钟前
十二个完成签到,获得积分10
5分钟前
5分钟前
葛二蛋完成签到,获得积分10
5分钟前
5分钟前
6分钟前
哈哈哈发布了新的文献求助10
6分钟前
xuzj完成签到 ,获得积分10
6分钟前
高分求助中
Licensing Deals in Pharmaceuticals 2019-2024 3000
Cognitive Paradigms in Knowledge Organisation 2000
Introduction to Spectroscopic Ellipsometry of Thin Film Materials Instrumentation, Data Analysis, and Applications 1800
Natural History of Mantodea 螳螂的自然史 1000
A Photographic Guide to Mantis of China 常见螳螂野外识别手册 800
How Maoism Was Made: Reconstructing China, 1949-1965 800
Barge Mooring (Oilfield Seamanship Series Volume 6) 600
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3314395
求助须知:如何正确求助?哪些是违规求助? 2946633
关于积分的说明 8531182
捐赠科研通 2622376
什么是DOI,文献DOI怎么找? 1434493
科研通“疑难数据库(出版商)”最低求助积分说明 665329
邀请新用户注册赠送积分活动 650881