自噬
内化
细胞生物学
细胞内
活性氧
聚对苯二甲酸乙二醇酯
化学
细胞
吸入
线粒体
细胞凋亡
生物
生物化学
材料科学
解剖
复合材料
作者
Balasubramanyam Annangi,Aliro Villacorta,Lourdes Vela,Alireza Tavakolpournegari,Ricard Marcos,Alba Hernández
标识
DOI:10.1016/j.etap.2023.104140
摘要
Since inhalation is a relevant exposure route, studies using appropriate micro/nanoplastic (MNPLs) models, representative targeted cells, and relevant biomarkers of effect are required. We have used lab-made polyethylene terephthalate (PET)NPLs obtained from PET plastic water bottles. Human primary nasal epithelial cells (HNEpCs) were used as a model of the first barrier of the respiratory system. Cell internalization and intracellular reactive oxygen species (iROS) induction, as well as the effects on mitochondria functionality and in the modulation of the autophagy pathway, were evaluated. The data indicated significant cellular uptake and increased levels of iROS. Furthermore, a loss of mitochondrial membrane potential was observed in the exposed cells. Regarding the effects on the autophagy pathway, PETNPLs exposure significantly increases LC3-II protein expression levels. PETNPLs exposure also induced significant increases in the expression of p62. This is the first study showing that true-to-life PETNPLs can alter the autophagy pathway in HNEpCs.
科研通智能强力驱动
Strongly Powered by AbleSci AI