核酸
微流控芯片
微流控
检出限
萃取(化学)
化学
色谱法
炸薯条
溶解
核酸定量
样品制备
洗脱
实验室晶片
纳米技术
材料科学
生物化学
计算机科学
电信
作者
Yaru Huang,Zehang Gao,Cong Ma,Yimeng Sun,Yuhang Huang,Chunping Jia,Jianlong Zhao,Shi‐Jin Feng
出处
期刊:Analyst
[The Royal Society of Chemistry]
日期:2023-01-01
卷期号:148 (12): 2758-2766
被引量:3
摘要
This paper introduces an enclosed microfluidic chip that integrates sample preparation and the chamber-based digital polymerase chain reaction (cdPCR). The sample preparation of the chip includes nucleic acid extraction and purification based on magnetic beads, which adsorb nucleic acids by moving around the reaction chambers to complete the reactions including lysis, washing, and elution. The cdPCR area of the chip consists of tens of thousands of regularly arranged microchambers. After the sample preparation processes are completed, the purified nucleic acid can be directly introduced into the microchambers for amplification and detection on the chip. The nucleic acid extraction performance and digital quantification performance of the system were examined using synthetic SARS-CoV-2 plasmid templates at concentrations ranging from 101-105 copies per μL. Further on, a simulated clinical sample was used to test the system, and the integrated chip was able to accurately detect SARS-CoV-2 virus particle samples doped with interference (saliva) with a detection limit of 10 copies per μL. This integrated system could provide a promising tool for point-of-care testing of pathogenic infections.
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