Rational and mechanistic approaches for improving biocatalyst performance

合理设计 生化工程 蛋白质工程 生物催化 定向进化 热稳定性 计算机科学 范围(计算机科学) 合成生物学 纳米技术 化学 工程类 材料科学 计算生物学 生物 催化作用 有机化学 生物化学 离子液体 程序设计语言 突变体 基因
作者
Aisaraphon Phintha,Pimchai Chaiyen
出处
期刊:Chem catalysis [Elsevier]
卷期号:2 (10): 2614-2643 被引量:4
标识
DOI:10.1016/j.checat.2022.09.026
摘要

Enzymes are biocatalysts capable of catalyzing reactions under environmentally friendly conditions and can be applied in a wide variety of applications. However, biocatalysts are often not stable or not functional under the conditions required by industries. Therefore, natural enzymes must be engineered to overcome these problems. Apart from the directed evolution strategy, rational, semi-rational, and mechanistic-based enzyme engineering have made significant contributions to the field. Here, we discuss recently developed approaches that have been shown to be useful in enhancing enzyme capabilities. These examples have improved the thermostability, activity, and solubility of diverse enzymes by utilizing rational-design and semi-rational-design protein engineering in combination with computational tools and mechanistic data. We discuss weaknesses, strengths, and challenging points for each approach and highlight recent successful cases of enzyme engineering by these methods. We also discuss the current and emerging trends in enzyme engineering and the challenging points remaining to be addressed. Biocatalysis is a green and sustainable tool for production of fine chemicals. Enzyme engineering is important for fine-tuning enzymes to contain properties suitable for industrial biocatalysis. In addition to the well-known directed evolution strategy, rational-design and semi-rational-design protein engineering, which can be performed with just a small-sized library for screening, have emerged as promising methods for increasing enzyme stability, catalytic efficiency, selectivity, and substrate scope. We have observed growth in computational tools as well as the use of mechanistic information to guide enzyme engineering. This knowledge is valuable for improving enzyme performance with limited resources and small-sized libraries, which should allow a wider community to carry out successful enzyme engineering programs. Enzymes are versatile biocatalysts that can be used in a variety of applications. However, their inherent properties do not always meet industrial needs, making enzyme engineering necessary. Besides directed evolution, rational and semi-rational enzyme engineering have made important contributions toward improvement of enzyme efficiency. Here, we discuss recent approaches in improving enzyme thermostability, catalytic activity, and solubility through rational and semi-rational design in combination with computational tools and mechanistic data. Upcoming trends in these approaches are also discussed.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
小成完成签到,获得积分10
1秒前
2秒前
2秒前
Link发布了新的文献求助10
2秒前
3秒前
4秒前
5秒前
yzw发布了新的文献求助10
5秒前
5秒前
英姑应助俏皮的飞烟采纳,获得10
6秒前
江湖小妖发布了新的文献求助10
7秒前
研友_VZG7GZ应助焦糖三分甜采纳,获得10
8秒前
周周完成签到,获得积分10
9秒前
12秒前
12秒前
高大的蚂蚁完成签到 ,获得积分10
13秒前
打打应助粗暴的海豚采纳,获得10
16秒前
Echan发布了新的文献求助10
18秒前
跳跳糖完成签到 ,获得积分10
21秒前
haikuotian举报成社长求助涉嫌违规
21秒前
24秒前
Delia完成签到,获得积分10
24秒前
领导范儿应助科研通管家采纳,获得30
25秒前
25秒前
科研通AI2S应助科研通管家采纳,获得10
25秒前
25秒前
smallxiao应助科研通管家采纳,获得10
26秒前
脑洞疼应助科研通管家采纳,获得10
26秒前
zpf应助科研通管家采纳,获得10
26秒前
科研通AI2S应助科研通管家采纳,获得10
26秒前
zpf应助科研通管家采纳,获得10
26秒前
Akim应助科研通管家采纳,获得10
26秒前
顾矜应助科研通管家采纳,获得10
26秒前
抹月批风完成签到,获得积分10
26秒前
越越给越越的求助进行了留言
27秒前
ok应助paulmichael采纳,获得10
28秒前
28秒前
29秒前
圈儿关注了科研通微信公众号
30秒前
高分求助中
Sustainability in Tides Chemistry 2000
The ACS Guide to Scholarly Communication 2000
Studien zur Ideengeschichte der Gesetzgebung 1000
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Threaded Harmony: A Sustainable Approach to Fashion 810
Pharmacogenomics: Applications to Patient Care, Third Edition 800
Free Will in the Flesh 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3081333
求助须知:如何正确求助?哪些是违规求助? 2733993
关于积分的说明 7531308
捐赠科研通 2383422
什么是DOI,文献DOI怎么找? 1263823
科研通“疑难数据库(出版商)”最低求助积分说明 612420
版权声明 597548