硫胺素
化学
葡萄糖醛酸盐
生物化学
炎症性肠病
微生物学
生物
内科学
医学
疾病
作者
Haochen Hui,Zhuoya Wang,Xuerong Zhao,Lina Xu,Lianhong Yin,Feifei Wang,Liping Qu,Jinyong Peng
标识
DOI:10.1016/j.jpha.2023.08.003
摘要
Inflammatory bowel disease (IBD) is a serious disorder, and exploration of active compounds to treat it is necessary. An acidic polysaccharide named SUSP-4 was purified from Selaginella uncinata (Desv.) Spring, which contained galacturonic acid, galactose, xylose, arabinose and rhamnose with the main chain structure of →4)-α-D-GalAp-(1→ and →6)-β-D-Galp-(1→ and the branched structure of →5)-α-L-Araf-(1→. Animal experiments showed that compared with Model group, SUSP-4 significantly improved body weight status, disease activity index (DAI), colonic shortening and histopathological damage and elevated Occludin and ZO-1 (Zonula occludens protein 1) expression in mice induced by dextran sulfate sodium salt (DSS). 16S rRNA sequencing indicated that SUSP-4 markedly downregulated the level of Akkermansia and Alistipes. Metabolomics results confirmed that SUSP-4 obviously elevated thiamine levels compared with Model mice by adjusting thiamine metabolism, which was further confirmed by a targeted metabolism study. Fecal transplantation experiments showed that SUSP-4 exerted an anti-IBD effect by altering the intestinal flora in mice. A mechanistic study showed that SUSP-4 markedly inhibited macrophage activation by decreasing the levels of phospho-nuclear factor kappa-B (P-P65) and cyclooxygenase-2 (COX-2) and elevating NF-E2-related factor 2 (Nrf2) levels compared with Model group. In conclusion, SUSP-4 affected thiamine metabolism by regulating Akkermania and inhibited macrophage activation to adjust NF-κB/Nrf2/COX-2-mediated inflammation and oxidative stress against IBD. This is the first time that plant polysaccharides have been shown to affect thiamine metabolism against IBD, showing great potential for in-depth research and development applications.
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