结肠炎
肠道菌群
橙皮素
毛螺菌科
溃疡性结肠炎
药理学
化学
免疫学
生物
抗氧化剂
医学
生物化学
厚壁菌
类黄酮
内科学
16S核糖体RNA
疾病
基因
作者
Jinzhi Wang,Yuanyuan Yao,Ting Yao,Qingmiao Shi,Yifan Zeng,Lanjuan Li
出处
期刊:Nutrients
[MDPI AG]
日期:2024-07-19
卷期号:16 (14): 2343-2343
被引量:4
摘要
Hesperetin (HT) is a type of citrus flavonoid with various pharmacological activities, including anti-tumor, anti-inflammation, antioxidant, and neuroprotective properties. However, the role and mechanism of HT in ulcerative colitis (UC) have been rarely studied. Our study aimed to uncover the beneficial effects of HT and its detailed mechanism in UC. Experimental colitis was induced by 2.5% dextran sodium sulfate (DSS) for seven days. HT ameliorated DSS-induced colitis in mice, showing marked improvement in weight loss, colon length, colonic pathological severity, and the levels of TNFα and IL6 in serum. A combination of informatics, network pharmacology, and molecular docking identified eight key targets and multi-pathways influenced by HT in UC. As a highlight, the experimental validation demonstrated that PTGS2, a marker of ferroptosis, along with other indicators of ferroptosis (such as ACSL4, Gpx4, and lipid peroxidation), were regulated by HT in vivo and in vitro. Additionally, the supplement of HT increased the diversity of gut microbiota, decreased the relative abundance of Proteobacteria and Gammaproteobacteria, and restored beneficial bacteria (Lachnospiraceae_NK4A136_group and Prevotellaceae_UCG-001). In conclusion, HT is an effective nutritional supplement against experimental colitis by suppressing ferroptosis and modulating gut microbiota.
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