Yi-Shen-Hua-Shi granule ameliorates diabetic kidney disease by the “gut-kidney axis”

内分泌学 内科学 糖尿病 颗粒(地质) 医学 生物 化学 古生物学
作者
Cong Han,Zhen Shen,Tao Cui,Shan-shan Ai,Ranran Gao,Yao Liu,Gui-yuan Sui,Hongzhen Hu,Wei Li
出处
期刊:Journal of Ethnopharmacology [Elsevier BV]
卷期号:307: 116257-116257 被引量:26
标识
DOI:10.1016/j.jep.2023.116257
摘要

Yi-Shen-Hua-Shi (YSHS) granule is an effective prescription widely used in traditional Chinese medicine to treat diabetic kidney disease (DKD), its exact efficacy in treating DKD has been confirmed but the underlying regulatory mechanism has not been fully elucidated.To explore the mechanism by which YSHS granule regulates intestinal flora and serum metabolites and then regulates renal mRNA expression through the "gut-kidney axis", so as to improve DKD.40 rats were divided into five groups: Normal group (N) (normal saline), model group (M) (STZ + normal saline), YSHS granule low-dose group (YL) (STZ + 2.27 g kg-1 d-1), YSHS granule high-dose group (YH) (STZ + 5.54g kg-1 d-1) and valsartan group (V) (STZ + 7.38mg kg-1 d-1). After 6 weeks, changes in blood glucose, blood lipids, and renal function related indexes were observed, as well as pathological changes in the kidney and colon. Intestinal microbiota was sequenced by 16S rDNA, serum differential metabolites were identified by LC-MS/MS, and renal differences in mRNA expression were observed by RNA-seq. Further, through the association analysis of intestinal differential microbiota, serum differential metabolites and kidney differential mRNAs, the target flora, target metabolites and target genes of YSHS granule were screened and verified, and the "gut-metabolism-transcription" co-expression network was constructed.In group M, blood glucose, blood lipid and proteinuria were increased, inflammation, oxidative stress and renal function were aggravated, with the proliferation of mesangial matrix, vacuolar degeneration of renal tubules, accumulation of collagen and lipid, and increased intestinal permeability, and YSHS granule and valsartan improved these disorders to varying degrees. High dose of YSHS granule improved the diversity and abundance of flora, decreased the F/B value, greatly increased the abundance of Lactobacillus and Lactobacillus_murinus, and decreased the abundance of Prevoella UCG_001. 14 target metabolites of YSHS granule were identified, which were mainly enriched in 20 KEGG pathways, such as Glycerophospholipid metabolism, Sphingolipid metabolism and Phenylalanine, tyrosine and tryptophan biosynthesis. 96 target mRNAs of YSHS granule were also identified. The enriched top 20 pathways were closely related to glucose and lipid metabolism, of which a total of 21 differential mRNAs were expressed. Further correlation analysis revealed that Lactobacillus, Lactobacillus_murinus and Prevotella UCG_001 were highly correlated with Glycerophospholipid metabolism, Sphingolipid metabolism and Phenylalanine, tyrosine and tryptophan biosynthesis pathways. At the same time, 6 pathways including Glycerophospholipid metabolism, Arachidonic acid metabolism, Purine metabolism, Primary bile acid biosynthesis, Ascorbate and aldarate metabolism and Galactose metabolism were co-enriched by the target metabolites and the target mRNAs of YSHS granule, including 7 differential metabolites such as phosphatidylethanolamine and 7 differential genes such as Adcy3. The 7 differential metabolites had high predictive value of AUC, and the validation of 7 differential genes were highly consistent with the sequencing results.YSHS granule could improve DKD through the "gut-kidney axis". Lactobacillus and Lactobacillus_murinus were the main driving forces. 6 pathways related to glucose and lipid metabolism, especially Glycerophospholipid metabolism, may be an important follow-up response and regulatory mechanism.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
DeepLearning发布了新的文献求助10
刚刚
SOL完成签到 ,获得积分10
15秒前
丝丢皮的完成签到 ,获得积分10
18秒前
t铁核桃1985完成签到 ,获得积分10
18秒前
丝丢皮得完成签到 ,获得积分10
24秒前
爆米花应助DeepLearning采纳,获得10
28秒前
铜豌豆完成签到 ,获得积分10
28秒前
cheng完成签到,获得积分10
35秒前
君看一叶舟完成签到 ,获得积分10
41秒前
在水一方应助科研通管家采纳,获得10
41秒前
42秒前
Chuang完成签到 ,获得积分10
56秒前
热心市民完成签到 ,获得积分10
1分钟前
吃不胖的魔芋丝完成签到 ,获得积分10
1分钟前
1分钟前
李东东完成签到 ,获得积分10
1分钟前
fsznc1完成签到 ,获得积分0
1分钟前
年轻的笙完成签到,获得积分10
1分钟前
嘻嘻完成签到 ,获得积分10
1分钟前
huiluowork完成签到 ,获得积分10
1分钟前
1分钟前
拾陆发布了新的文献求助10
1分钟前
乔杰完成签到 ,获得积分10
1分钟前
ramsey33完成签到 ,获得积分10
1分钟前
小静完成签到 ,获得积分10
1分钟前
默笙完成签到 ,获得积分10
1分钟前
小乙猪完成签到 ,获得积分0
1分钟前
赵帅完成签到 ,获得积分10
1分钟前
1分钟前
西扬完成签到 ,获得积分10
1分钟前
1分钟前
Ray完成签到 ,获得积分10
1分钟前
yyds完成签到,获得积分10
1分钟前
DeepLearning发布了新的文献求助10
1分钟前
1分钟前
柚C美式完成签到 ,获得积分10
1分钟前
kumo完成签到 ,获得积分10
1分钟前
旺旺大礼包完成签到,获得积分10
2分钟前
小康学弟完成签到 ,获得积分10
2分钟前
大个应助DeepLearning采纳,获得10
2分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990835
求助须知:如何正确求助?哪些是违规求助? 3532241
关于积分的说明 11256614
捐赠科研通 3271100
什么是DOI,文献DOI怎么找? 1805229
邀请新用户注册赠送积分活动 882302
科研通“疑难数据库(出版商)”最低求助积分说明 809236