循环肿瘤细胞
分离(微生物学)
转移
磁性纳米粒子
肽
化学
癌症
生物物理学
材料科学
医学
纳米技术
纳米颗粒
生物化学
生物
微生物学
内科学
作者
Linling Bai,Yimeng Du,Jiaxi Peng,Yi Liu,Yanmei Wang,Yanlian Yang,Chen Wang
摘要
Detection of rare circulating tumor cells (CTCs) in the peripheral blood of metastatic cancer patients has shown promise for improved diagnosis, staging and prognosis of cancers. The epithelial cell adhesion molecule (EpCAM) has been revealed to be over-expressed in CTCs while it is absent in normal blood cells and has been used as an efficient diagnosis and therapeutic target on CTCs, especially in CTC isolation and detection. Most of the CTC isolation techniques are based on nanomaterials or nanostructured surfaces functionalized with the EpCAM antibody. Herein, instead of anti-EpCAM, we report a new CTC isolation method with high efficiency by using the EpCAM recognition peptide functionalized iron oxide magnetic nanoparticles (MNPs) (Pep@MNPs). The de novo designed peptide, Pep10, with comparable binding affinity KD (1.98 × 10-9 mol L-1) to that of the anti-EpCAM (2.69 × 10-10 mol L-1) is attached onto MNPs via biotin-avidin interaction. We demonstrate that Pep10@MNPs (200 nm) have the comparable capture efficiency (reaching above 90%) and purity (reaching above 93%) to anti-EpCAM@MNPs for breast, prostate and liver cancers from spiked human blood. Furthermore, the captured cells still maintain viability for further molecular biological analysis with this method. The peptide-based CTC isolation method could be beneficial for cancer prognosis and metastasis prevention by increasing the stability and reproducibility.
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