Expression and characterization of human bifunctional peptidylglycine α-amidating monooxygenase

双功能 化学 中国仓鼠卵巢细胞 苯乙二醛 单加氧酶 磷酸果糖激酶2 生物化学 立体化学 催化作用 氨基酸 细胞色素P450 精氨酸 受体
作者
Manabu Satani,Ken­ichi Takahashi,Hiroshi Sakamoto,Saori Harada,Yasuhiko Kaida,Masato Noguchi
出处
期刊:Protein Expression and Purification [Elsevier]
卷期号:28 (2): 293-302 被引量:21
标识
DOI:10.1016/s1046-5928(02)00684-8
摘要

We report the purification and characterization of human bifunctional peptidylglycine alpha-amidating monooxygenase (the bifunctional PAM) expressed in Chinese hamster ovary cells. PAM is in charge of the formation of the C-terminal amides of biologically active peptides. The bifunctional PAM possesses two catalytic domains in a single polypeptide, peptidylglycine alpha-hydroxylating monooxygenase (PHM, EC 1.14.17.3) and peptidylamidoglycolate lyase (PAL, EC 4.3.2.5). By introducing a stop codon at 835 Glu, we were able to eliminate the membrane-spanning domain in the C-terminal region and succeeded in purifying a soluble form of bifunctional PAM that was secreted into the medium. Through a three-step purification procedure, we obtained 0.3mg of the purified PAM, which showed a single band at 91 kDa on SDS-PAGE, from 1L of monolayer culture medium. Metals contained in the purified PAM were analyzed and chemical modifications were performed to gain insight into the mechanism of the PAL reaction. Inductively coupled plasma detected 0.62 mol of Zn(2+) and 1.25 mol of Cu(2+) per mol of bifunctional PAM. Further, the addition of 1mM EDTA reduced the PAL activity by about 50%, but the decreased activity was recovered by the addition of an excess amount of Zn(2+). In a series of chemical modifications, phenylglyoxal almost completely eliminated the PAL activity and diethyl pyrocarbonate suppressed activity by more than 70%. These findings implied that Arg and His residues might play crucial roles during catalysis.
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