A Bioinspired Material for Bone Tissue Regeneration: The Use of Ganoderma sessile Mycelium as a Microstructure Director

first_page Download PDF settings Order Article Reprints Font Type: Arial Georgia Verdana Font Size: Aa Aa Aa Line Spacing:    Column Width:    Background: Open AccessAbstract A Bioinspired Material for Bone Tissue Regeneration: The Use of Ganoderma sessile Mycelium as a Microstructure Director † by Noelia Laura D'ElíaNoelia Laura D'Elía SciProfiles Scilit Preprints.org Google Scholar 1,*, Javier SartuquiJavier Sartuqui SciProfiles Scilit Preprints.org Google Scholar 1, Damian PlacenteDamian Placente SciProfiles Scilit Preprints.org Google Scholar 1, Pablo PostemskyPablo Postemsky SciProfiles Scilit Preprints.org Google Scholar 2 and Paula Verónica MessinaPaula Verónica Messina SciProfiles Scilit Preprints.org Google Scholar 1 1 INQUISUR—CONICET, Departamento de Química, Universidad Nacional del Sur, Bahía Blanca B8000, Argentina 2 Laboratory of Biotechnology of Edible and Medicinal Mushrooms, Centro de Recursos Naturales Renovables de la Zona Semiárida (CERZOS-UNS/CONICET), Camino de La Carrindaga Km7, Bahía Blanca B8000, Argentina * Author to whom correspondence should be addressed. † Presented at the 1st International Online Conference on Biomimetics (IOCB 2024), 15–17 May 2024; Available online: https://sciforum.net/event/IOCB2024. Proceedings 2024, 107(1), 2; https://doi.org/10.3390/proceedings2024107002 Published: 15 May 2024 Download keyboard_arrow_down Download PDF Download PDF with Cover Download XML Download Epub Download Supplementary Material Versions Notes Keywords: mycelium; alginate; hydroxyapatite; Ganoderma Introduction. The development of new strategies to repair large segmental bone defects is currently an ongoing challenge all around the world, and biomaterials suitable for dealing with this are in high demand. An important aim in this field is to simultaneously meet both the mechanical and biological requirements of the implant site.Methodology. In this study, we propose obtaining a bioinspired bone tissue substitute using the stiff and modulable mycelium of Ganoderma sessile. The mycelium was cultured on a substrate composed of alginate crosslinked by hydroxyapatite nanoparticles (ALG-HAn), with in vitro osteogenic properties previously verified by the authors. Then, the mycelium was inactivated and sterilized by autoclaving to obtain the final biomaterial.Results. Using scanning electron microscopy (SEM), it was possible to confirm that the mycelium acts as a directing agent of the biomaterial microstructure. The mycelium colonized the ALG-HAn substrate, leading to the formation of a trabecular bone-like network with a hierarchical structure. Moreover, static water contact angle assays demonstrated that the presence of ALG in the membranes significantly reduced the hydrophobicity of the biomaterials. Finally, to test the interaction between blood cells and biomaterial, we verify the lack of hemolysis in plasma samples.Conclusions. The promising results of this work will provide a new perspective for the future development of mycelium-based biomaterials applied for bone tissue regeneration. Supplementary MaterialsThe following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/proceedings2024107002/s1.Author ContributionsConceptualization, N.L.D. and P.P.; methodology, N.L.D., P.P., J.S. and D.P.; formal analysis, N.L.D., P.P., J.S. and D.P.; resources, P.V.M. and P.P.; writing—original draft preparation, N.L.D. and J.S.; writing—review and editing, N.L.D., J.S. and P.P.; supervision, P.V.M., P.P. and N.L.D.; project administration, P.V.M. and P.P.; funding acquisition, P.V.M. and P.P. All authors have read and agreed to the published version of the manuscript.FundingThis research was funded by Universidad Nacional del Sur (UNS) grant number PGI 24/131; Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT) grant number PICT-2021-I-A-00108 and PICT 2020-3527, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) grant number PIP-11220210100126CO and ImpaCT.AR program grant number Impactar D76. D.P. has a postdoctoral fellowships of CONICET; J.S., N.L.D., P.P. and P.V.M. are researchers of CONICET.Institutional Review Board StatementFresh whole-blood samples were obtained from the subjects according to guidelines of Good Clinical Practices (ICH GCP Guidelines) of The Code of Ethics of the World Medical Association (the tenets of the Declaration of Helsinki) and Argentine National disposition ANMAT 6677/10 and Buenos Aires provincial law (no. 11044).Informed Consent StatementInformed consent was obtained from all blood donors. Neither case details nor other personal information has been obtained from whole-blood samples.Data Availability StatementNot applicable.Conflicts of InterestThe authors declare no conflict of interest.Disclaimer/Publisher's Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Share and Cite MDPI and ACS Style D'Elía, N.L.; Sartuqui, J.; Placente, D.; Postemsky, P.; Messina, P.V. A Bioinspired Material for Bone Tissue Regeneration: The Use of Ganoderma sessile Mycelium as a Microstructure Director. Proceedings 2024, 107, 2. https://doi.org/10.3390/proceedings2024107002 AMA Style D'Elía NL, Sartuqui J, Placente D, Postemsky P, Messina PV. A Bioinspired Material for Bone Tissue Regeneration: The Use of Ganoderma sessile Mycelium as a Microstructure Director. Proceedings. 2024; 107(1):2. https://doi.org/10.3390/proceedings2024107002 Chicago/Turabian Style D'Elía, Noelia Laura, Javier Sartuqui, Damian Placente, Pablo Postemsky, and Paula Verónica Messina. 2024. "A Bioinspired Material for Bone Tissue Regeneration: The Use of Ganoderma sessile Mycelium as a Microstructure Director" Proceedings 107, no. 1: 2. https://doi.org/10.3390/proceedings2024107002 Article Metrics No No Article Access Statistics Multiple requests from the same IP address are counted as one view.