Hypoxic–Normoxic Crosstalk Activates Pro‐Inflammatory Signaling in Human Cardiac Fibroblasts and Myocytes in a Post‐Infarct Myocardium on a Chip

Abstract Myocardial infarctions locally deprive myocardium of oxygenated blood and cause immediate cardiac myocyte necrosis. Irreparable myocardium is then replaced with a scar through a dynamic repair process that is an interplay between hypoxic cells of the infarct zone and normoxic cells of adjacent healthy myocardium. In many cases, unresolved inflammation or fibrosis occurs for reasons that are incompletely understood, increasing the risk of heart failure. Crosstalk between hypoxic and normoxic cardiac cells is hypothesized to regulate mechanisms of repair after a myocardial infarction. To test this hypothesis, microfluidic devices are fabricated on 3D printed templates for co‐culturing hypoxic and normoxic cardiac cells. This system demonstrates that hypoxia drives human cardiac fibroblasts toward glycolysis and a pro‐fibrotic phenotype, similar to the anti‐inflammatory phase of wound healing. Co‐culture with normoxic fibroblasts uniquely upregulates pro‐inflammatory signaling in hypoxic fibroblasts, including increased secretion of tumor necrosis factor alpha (TNF‐α). In co‐culture with hypoxic fibroblasts, normoxic human induced pluripotent stem cell (hiPSC)‐derived cardiac myocytes also increase pro‐inflammatory signaling, including upregulation of interleukin 6 (IL‐6) family signaling pathway and increased expression of IL‐6 receptor. Together, these data suggest that crosstalk between hypoxic fibroblasts and normoxic cardiac cells uniquely activates phenotypes that resemble the initial pro‐inflammatory phase of post‐infarct wound healing.