癌症
免疫疗法
CXCL13型
医学
癌症免疫疗法
癌症研究
CD8型
免疫学
肿瘤科
抗原
内科学
免疫系统
趋化因子受体
趋化因子
作者
Jiawei Wang,Yuan Liang,Ao X,Jian Xiao,Xinyu Zhao,Shuqing Cao,Pengyu Li,Jiahao Dong,Yuan Li,Zekuan Xu,Li Yang
标识
DOI:10.1136/jitc-2024-009603
摘要
Background Stage IV gastric cancer is a highly heterogeneous and lethal tumor with few therapeutic strategies. The combination of programmed cell death protein 1 inhibitors and chemotherapy is currently the standard frontline treatment regimen for advanced gastric cancer. Nevertheless, it remains a great challenge to screen the beneficiaries of immunochemotherapy and expand indications for this treatment regimen. Methods We conducted a pathological assessment to ascertain the importance of tertiary lymphoid structures based on the tissue samples collected from patients with stage IV gastric cancer (n=15) both prior to and following immunochemotherapy treatment. Additionally, we used spatial (n=10) and single-cell transcriptional analysis (n=97) to investigate the key regulators of tertiary lymphoid structures (TLSs). Multiplex immunofluorescence and image analysis (n=34) were performed to explore the association between tumor-infiltrating CXCL13 + CD160 + CD8 + T cells and TLSs. The relationship between CXCL13 + CD160 + CD8 + T cells and the responsiveness to immunotherapy was also evaluated by multiplex immunofluorescence and image analysis approaches (n=15). Furthermore, we explored the intrinsic characteristics of CXCL13 + CD160 + CD8 + T cells through various experimental techniques, including quantitative reverse transcription-PCR, western blot, and flow cytometry. Results We found that responders exhibited higher levels of TLSs and CXCL13 + CD160 + CD8 + T cells in biopsy tissues prior to immunochemotherapy compared with non-responders. Following conversion therapy, responders also had a higher percentage of mature TLSs and a higher number of CXCL13 + CD160 + CD8 + T cells in surgical resections. Moreover, we discovered that vitamin B 6 in CD160 + CD8 + T cells could reduce the ubiquitination modification of HIF-1α by MDM2, thereby attenuating the degradation of HIF-1α. Consequently, this led to the transcriptional upregulation of CXCL13 expression, facilitating the recruitment of CXCR5 + B cells and the formation of TLSs. Conclusion The number and maturity of TLSs, along with the extent of CXCL13 + CD160 + CD8 + T-cell infiltration, might function as potential indicators for assessing the effectiveness of immunotherapy in treating gastric malignancies. Furthermore, our research suggests that vitamin B 6 could enhance the secretion of CXCL13 by CD160 + CD8 + T cells by reducing the degradation of HIF-1α. Additionally, we demonstrate that vitamin B 6 supplementation or targeting pyridoxal kinase could substantially improve the efficacy of immunotherapies for gastric cancer.
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