细胞外基质
纤维连接蛋白
基质凝胶
再生医学
脚手架
材料科学
纳米技术
层粘连蛋白
生物物理学
细胞
细胞生物学
化学
生物
计算机科学
生物化学
数据库
作者
Sophia J. Bailey,Erik Hopkins,Naomi Baxter,Isobel Whitehead,Javier Read de Alaniz,Maxwell Z. Wilson
标识
DOI:10.1002/adma.202303453
摘要
Abstract Strategies that mimic the spatial complexity of natural tissues can provide cellular scaffolds to probe fundamental questions in cell biology and offer new materials for regenerative medicine. Here, the authors demonstrate a light‐guided patterning platform that uses natural engineered extracellular matrix (ECM) proteins as a substrate to program cellular behaviors. A photocaged diene which undergoes Diels–Alder‐based click chemistry upon uncaging with 365 nm light is utilized. By interfacing with commercially available maleimide dienophiles, patterning of common ECM proteins (collagen, fibronectin Matrigel, laminin) with readily purchased functional small molecules and growth factors is achieved. Finally, the use of this platform to spatially control ERK activity and migration in mammalian cells is highlighted, demonstrating programmable cell behavior through patterned chemical modification of natural ECM.
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