带状突触
视网膜
斑马鱼
神经科学
内丛状层
胞吐
生物
突触
视网膜
细胞生物学
突触小泡
解剖
内分泌学
生物化学
小泡
遗传学
膜
分泌物
基因
作者
Abhishek P. Shrestha,Nirujan Rameshkumar,Johane M. Boff,Rhea Rajmanna,Thadshayini Chandrasegaran,Courtney Frederick,David Zenisek,Thirumalini Vaithianathan
出处
期刊:Cells
[MDPI AG]
日期:2023-09-29
卷期号:12 (19): 2385-2385
标识
DOI:10.3390/cells12192385
摘要
The global health concern posed by age-related visual impairment highlights the need for further research focused on the visual changes that occur during the process of aging. To date, multiple sensory alterations related to aging have been identified, including morphological and functional changes in inner hair cochlear cells, photoreceptors, and retinal ganglion cells. While some age-related morphological changes are known to occur in rod bipolar cells in the retina, their effects on these cells and on their connection to other cells via ribbon synapses remain elusive. To investigate the effects of aging on rod bipolar cells and their ribbon synapses, we compared synaptic calcium currents, calcium dynamics, and exocytosis in zebrafish (Danio rerio) that were middle-aged (MA,18 months) or old-aged (OA, 36 months). The bipolar cell terminal in OA zebrafish exhibited a two-fold reduction in number of synaptic ribbons, an increased ribbon length, and a decrease in local Ca2+ signals at the tested ribbon location, with little change in the overall magnitude of the calcium current or exocytosis in response to brief pulses. Staining of the synaptic ribbons with antibodies specific for PKCa revealed shortening of the inner nuclear and plexiform layers (INL and IPL). These findings shed light on age-related changes in the retina that are related to synaptic ribbons and calcium signals.
科研通智能强力驱动
Strongly Powered by AbleSci AI