Lewis Acid‐Catalyzed Substituted spiro 4‐thia‐1,2,6,9 tetraazaspiro[4.4]non‐2‐ene‐7,8‐diones via 1,3‐dipolar [3+2]cycloaddition

Abstract A series of substituted spiro 4‐thia‐1,2,6,9‐tetraazaspiro[4.4]non‐2‐ene‐7,8‐diones were synthesized with good yields through 1,3‐dipolar substituted hydrazonoylchloride's substituted 2‐thioxoimidazolidin‐4,5‐diones under mild conditions as described. This switchable C−S and C−N bond connection with simple execution and broad substrate scope. In this study, the synthesized compounds 3 a – q were screened against four distinct cancer cell lines (PANC‐1, MIA PaCa‐2, MDA MB231 and DU145) along with a standard cell line HEK‐293 , to determine their in vitro anticancer activities, and assessed using the Sulphorhodamine B assay to determine their viability