Evaluation of Mearnsitrin on cardiomyocyte apoptosis induced by doxorubicin in H9c2 Cardiomyoblast Cells

Objective: Doxorubicin (Dox) is a type of chemotherapy drug it slows or stops the growth of cancer cells by blocking an enzyme called topoisomerase-2 and produces cardiotoxicity by inducing apoptosis.Mearnsitrin is a flavonoid compound from the stems of DC and is known to be a natural antioxidant.The study is carried out Emilia sonchifolia the evaluation of mearnsitrin on cardiomyocyte apoptosis induced by doxorubicin in H9c2 Cardiomyoblast Cells.Materials and methods: Rat cardiac H9C2 cells were cultured in DMEM supplemented with 10% fetal bovine serum, 100 U/ml of penicillin, 100 μg/ml of streptomycin and 5% CO2 at 37 °C.A modified MTT assay was used to determine cell viability.Quantitative real time RT-PCR was used to evaluate the expression of Bcl-2 in cardiomyocytes.No toxicity observed when the cells exposed for 1 hour to different concentrations of Results: mearnsitrin, but pretreatment of cells with mearnsitrin increased cytotoxicity of DOX in a dose dependent manner.RT-PCR analysis showed that mearnsitrin significant mRNA gene expression of Bcl2 compared to cells treated with DOX alone.The data indicated that subtoxic concentrations of mearnsitrin sensitize H9c2 cells to Conclusion: DOX-induce apoptosis.These results suggest that the use of mearnsitrin in combination with DOX reduces the cardiomyocyte apoptosis induced in H9c2 cardiomyoblast cells than DOX alone.