CD177+ neutrophils as functionally activated neutrophils negatively regulate IBD

中性粒细胞胞外陷阱 免疫学 促炎细胞因子 发病机制 人口 白细胞介素8 炎症 髓过氧化物酶 吞噬作用 肠粘膜 医学 环境卫生 内科学
作者
Guangxi Zhou,Yu Lin,Leilei Fang,Wenjing Yang,Tianming Yu,Yinglei Miao,Minhu Chen,Kaichun Wu,Feidi Chen,Yingzi Cong,Zhanju Liu
出处
期刊:Gut [BMJ]
卷期号:67 (6): 1052-1063 被引量:178
标识
DOI:10.1136/gutjnl-2016-313535
摘要

Background Neutrophils are accumulated in inflamed mucosa of IBD and play an important role in the pathogenesis. CD177 is expressed in neutrophils specifically and upregulated during inflammation. However, the role of CD177 + neutrophils in pathogenesis of IBD remains elusive. Materials and methods Expression of CD177 was analysed in peripheral blood and intestinal mucosa from patients with IBD using quantitative RT-PCR, flow cytometry and immunohistochemistry. CD177 + and CD177 − neutrophils were isolated to determine gene differences by RNA sequencing. Colitis was established in CD177 −/− and wild-type mice in response to dextran sulfate sodium (DSS) insults to determine the role of CD177 + neutrophils in IBD. Results CD177 + neutrophils were markedly increased in peripheral blood and inflamed mucosa from patients with active IBD compared with healthy controls. RNA sequencing revealed that differential gene expression between CD177 + and CD177 − neutrophils from patients with IBD was associated with response to bacterial defence, hydrogen peroxide and reactive oxygen species (ROS). CD177 + neutrophils produced lower levels of proinflammatory cytokines (ie, interferon-γ, interleukin (IL)-6, IL-17A), but higher levels of IL-22 and transforming growth factor-β, and exhibited increased bactericidal activities (ie, ROS, antimicrobial peptides, neutrophil extracellular trap) compared with CD177 − subset. CD177 −/− mice developed more severe colitis on DSS insults compared with wild-type mice. Moreover, CD177 deficiency led to compromised intestinal barrier and impaired antibacterial immunity through decreased production of IL-22 by CD177 − neutrophils. Conclusions CD177 + neutrophils represent functionally activated population and play a protective role in IBD through increased bactericidal activity and IL-22 production. Targeting CD177 + neutrophils may be beneficial for treatment of IBD.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Cuz完成签到,获得积分10
1秒前
香蕉觅云应助Moving_Dr采纳,获得10
1秒前
科研狗完成签到 ,获得积分20
1秒前
yu完成签到,获得积分10
2秒前
狂野大雄鹰完成签到 ,获得积分10
3秒前
Singularity发布了新的文献求助10
3秒前
4秒前
shelter应助xiazhq采纳,获得10
4秒前
5秒前
lin发布了新的文献求助10
5秒前
爆米花应助怡然珊采纳,获得10
5秒前
bkagyin应助dahong采纳,获得80
5秒前
wushuimei完成签到 ,获得积分10
6秒前
绒绒发布了新的文献求助10
7秒前
香蕉觅云应助哈哈哈采纳,获得10
7秒前
QQ发布了新的文献求助10
8秒前
Lou完成签到,获得积分10
12秒前
wbh发布了新的文献求助20
12秒前
14秒前
Lucas应助科研通管家采纳,获得10
14秒前
田様应助科研通管家采纳,获得10
14秒前
大个应助科研通管家采纳,获得10
14秒前
今后应助故意的乐菱采纳,获得10
15秒前
吴也行完成签到,获得积分10
15秒前
JamesPei应助keyan采纳,获得10
16秒前
李李发布了新的文献求助30
17秒前
yoruyik完成签到 ,获得积分10
19秒前
WWZZSS发布了新的文献求助20
20秒前
勤劳的斑马完成签到,获得积分10
20秒前
21秒前
22秒前
共享精神应助wbh采纳,获得10
23秒前
万能图书馆应助Singularity采纳,获得10
24秒前
天天快乐应助WWW采纳,获得10
24秒前
25秒前
26秒前
cy完成签到,获得积分10
27秒前
研友_ZzwoR8发布了新的文献求助10
28秒前
周老八发布了新的文献求助10
28秒前
29秒前
高分求助中
The ACS Guide to Scholarly Communication 2500
Sustainability in Tides Chemistry 2000
Microlepidoptera Palaearctica, Volumes 1 and 3 - 13 (12-Volume Set) [German] 1122
The Data Economy: Tools and Applications 1000
Diamonds: Properties, Synthesis and Applications 800
PraxisRatgeber Mantiden., faszinierende Lauerjäger. – Buch gebraucht kaufen 700
Mantiden - Faszinierende Lauerjäger – Buch gebraucht kaufen 700
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3096435
求助须知:如何正确求助?哪些是违规求助? 2748408
关于积分的说明 7599570
捐赠科研通 2400094
什么是DOI,文献DOI怎么找? 1273377
科研通“疑难数据库(出版商)”最低求助积分说明 615726
版权声明 598973