抗坏血酸
检出限
催化作用
化学
吸光度
吸附
基质(水族馆)
溶剂
人工酶
金属
金属有机骨架
线性范围
生物传感器
无机化学
过氧化物酶
核化学
酶
色谱法
有机化学
生物化学
地质学
食品科学
海洋学
作者
Cunji Gao,Hongmei Zhu,Jia Chen,Hongdeng Qiu
标识
DOI:10.1016/j.cclet.2017.02.011
摘要
In this work, a metal-organic frameworks material MIL-88 was prepared easily using solvent-thermal method, and was first found to have catalytic activities similar to those of biological enzymes such as catalase and peroxidase. The material was characterized by XRD, SEM, TEM, EDX, FT-IR techniques and an N2 adsorption method. It exhibited peroxidase-like activity through catalytic oxidation of the peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H2O2, producing a blue-colored solution. Under optimal conditions, the absorbance at 652 nm is linearly correlated with the concentration of H2O2 from 2.0 × 10−6 mol/L to 2.03 × 10−5 mol/L (R2 = 0.981) with a detection limit of 5.62 × 10−7 mol/L (S/N = 3). More importantly, a sensitive and selective method for ascorbic acid detection was developed using this material as a catalyst. The analytical method for ascorbic acid detection was observed to have a linear range from 2.57 × 10−6 mol/L to 1.01 × 10−5 mol/L (R2 = 0.989) with a detection limit of 1.03 × 10−6 mol/L (S/N = 3). This work suggests MOFs have advantages of preparing biomimetic catalysts and extends applications of the functional MOFs in the field of biosensor.
科研通智能强力驱动
Strongly Powered by AbleSci AI