In Arabidopsis, low blue light enhances phototropism by releasing cryptochrome 1-mediated inhibition ofPIF4expression

向光性 光敏色素 拟南芥 隐色素 黄化 避光 下胚轴 嗜光蛋白 拟南芥 生长素 蓝光 远红色 生物 植物 细胞生物学 突变体 生物物理学 红灯 物理 生物化学 基因 生物钟 光电子学
作者
Alessandra Boccaccini,Martina Legris,Johanna Krahmer,Laure Allenbach Petrolati,Anupama Goyal,Carlos S. Galván-Ampudia,Teva Vernoux,Elisabeth Karayekov,Jorge J. Casal,Christian Fankhauser
标识
DOI:10.1101/2020.02.28.969725
摘要

Abstract Shade-avoiding plants including Arabidopsis thaliana display a number of growth responses elicited by shade cues including elongation of stem-like structures and repositioning of leaves. Shade also promotes phototropism of de-etiolated seedlings through repression of phytochrome B (phyB) presumably to enhance capture of unfiltered sunlight. Light cues indicative of shade include a reduction in the blue and red portions of the solar spectrum and a low red to far-red ratio. Here we show that in Arabidopsis seedlings both low blue and a low red to far-red ratio are required to rapidly enhance phototropism. However, prolonged low blue treatments through reduced cryptochrome 1 (cry1) activation are sufficient to promote phototropism. The enhanced phototropic response of cry1 mutants in the lab and in response to natural canopies depends on PHYTOCHROME INTERACTING FACTORs ( PIFs ). In favorable light conditions, cry1 limits the expression of PIF4 while in low blue light PIF4 expression increases, which contributes to phototropic enhancement. The analysis of a quantitative DII auxin reporter indicates that low blue light leads to enhanced auxin levels in the hypocotyl and, upon phototropic stimulation, a steeper auxin gradient across the hypocotyl. We conclude that phototropic enhancement by canopy shade results from the combined activities of phytochrome B and cry1 that converge on PIF regulation. ONE SENTENCE SUMMARY The persistent depletion of blue light in natural canopy shade relieves the inhibitory effect of cryptochrome 1 on PIF4, enhancing phototropism in de-etiolated Arabidopsis seedlings. Financial support This work was supported by the University of Lausanne and a grant from the Swiss National Science foundation (n° 310030B_179558 to C.F.); Human Frontier Science Program organization (HFSP) Grant RPG0054-2013, ANR-12-BSV6-0005 grant (AuxiFlo) to T.V.; The University of Buenos Aires (Grant 20020100100437 to J. J. C.), and Agencia Nacional de Promoción Científica y Tecnológica of Argentina (Grant PICT-2018-01695 to J. J. C.). Alessandra Boccaccini and Martina Legris are funded by Marie Curie fellowships H2020-MSCA-IF-2017 grants CRoSh 796283 and Flat-Leaf 796443 respectively.
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