生物分子
材料科学
灵敏度(控制系统)
纳米技术
石墨烯
电极
碳纳米管
分析物
核酸
生物传感器
化学
色谱法
电子工程
生物化学
工程类
物理化学
作者
Jing Bao,Xiaopei Qiu,Deqiang Wang,Huisi Yang,Jiaying Zhao,Yanli Qi,Liangliang Zhang,Xiaohong Chen,Mei Yang,Wei Gu,Danqun Huo,Yang Luo,Changjun Hou
标识
DOI:10.1002/adfm.202006521
摘要
Abstract Despite numerous efforts, the accurate determination of trace biomolecules with zeptomolar sensitivity remains elusive. Here, a 3D carbon nanomaze (CAM) electrode for the ultrasensitive detection of trace biomolecules such as nucleic acids, proteins, and extracellular vesicles is reported. The CAM electrode consists of an interlaced carbon fiber array on which intercrossed graphene sheets are vertically tethered in situ, permitting local confinement of trace molecules to increase molecular hybridization efficiency. Furthermore, a self‐assembled DNA tetrahedron array adopts a rigid spatial conformation to guarantee the controllable arrangement of immobilized biological probes, facilitating analytical sensitivity and reproducibility. In a proof‐of‐concept experiment on detecting microRNA‐155, a linearity of 0.1 aM to 100 nM and a sensitivity of 0.023 aM (23 zM) are achieved. With the optimal parameters, the proposed nanoelectrode demonstrates encouraging consistency with quantitative real‐time polymerase chain reaction during clinical sample detection. Through simple functionalization by appending various biomolecular probes of interest, the developed CAM platform with ultrahigh sensitivity can be exploited as a versatile tool in environmental, chemistry, biology, and healthcare fields.
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