Tantalum Oxide Nanoparticle-Based Mass Tag for Mass Cytometry

化学 结合 叠氮化物 组合化学 胺气处理 纳米颗粒 纳米技术 材料科学 有机化学 数学 数学分析
作者
Yefeng Zhang,Nick Zabinyakov,Daniel Majonis,Alexandre Bouzekri,Olga Ornatsky,Vladimir Baranov,Mitchell A. Winnik
出处
期刊:Analytical Chemistry [American Chemical Society]
卷期号:92 (8): 5741-5749 被引量:19
标识
DOI:10.1021/acs.analchem.9b04970
摘要

Mass cytometry (MC) is a bioanalytical technique that uses metal-tagged antibodies (Abs) for high-dimensional single-cell immunoassays. Currently, this technology can measure over 40 parameters simultaneously on individual cells using metal-chelating polymer (MCP) based reagents. However, MC can in principle detect up to 135 parameters with the development of new elemental mass tags. Here we report the development of a tantalum oxide nanoparticle (NP)-based mass tag for MC immunoassays. Uniform-sized amine-functionalized tantalum oxide NPs (d ∼ 5.7 nm) were synthesized via a one-pot two-step reverse microemulsion method. These amine-functionalized NPs were further modified with azide groups by reacting with azide-PEG2k succinimidyl carboxymethyl ester (NHS-PEG2k-N3) cross-linkers. The Ab-NP conjugates were prepared by reacting azide-functionalized NPs with dibenzocyclooctyne (DBCO)-functionalized primary or secondary Abs (DBCO-Ab) followed by fast protein size exclusion liquid chromatography (FPLC) purification. Three Ab-NP conjugates (TaO2-PEG2k-goat antimouse, TaO2-PEG2k-CD25, TaO2-PEG2k-CD196) were fabricated and tested in MC immunoassays. For the TaO2-PEG2k-goat antimouse conjugate, we showed that it can effectively detect abundant CD20 biomarkers on Ramos cells. For TaO2-PEG2k-CD25 and TaO2-PEG2k-CD196 conjugates, we demonstrated that these Ab-NP conjugates could be integrated into the commercial Ab staining panels for high-dimensional single-cell immune profiling of human peripheral blood mononuclear cells.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
mizo木子李发布了新的文献求助10
刚刚
NexusExplorer应助breen采纳,获得10
2秒前
DrW先生完成签到,获得积分10
2秒前
shuangfeng1853完成签到 ,获得积分10
2秒前
蜗牛完成签到,获得积分10
3秒前
4秒前
科研通AI2S应助xiaoliu采纳,获得10
4秒前
4秒前
完美世界应助晚风采纳,获得10
5秒前
Mp4发布了新的文献求助10
5秒前
充电宝应助Aurora采纳,获得10
6秒前
6秒前
wang完成签到,获得积分20
6秒前
小呆呆发布了新的文献求助10
7秒前
Owen应助rainbow采纳,获得10
7秒前
7秒前
Lucas应助自觉无心采纳,获得10
9秒前
CipherSage应助zyt采纳,获得10
10秒前
10秒前
桐桐应助yuyu采纳,获得10
11秒前
rush完成签到,获得积分10
12秒前
13秒前
龙龙ff11_完成签到,获得积分10
13秒前
CSS发布了新的文献求助10
13秒前
张sire发布了新的文献求助10
14秒前
W29完成签到,获得积分10
14秒前
15秒前
bkagyin应助mizo木子李采纳,获得10
16秒前
晚风发布了新的文献求助10
16秒前
我超爱cs完成签到,获得积分10
17秒前
wojwosjns完成签到,获得积分10
17秒前
18秒前
18秒前
19秒前
19秒前
19秒前
20秒前
K. G.发布了新的文献求助10
20秒前
两个我完成签到,获得积分10
22秒前
科研桃完成签到,获得积分20
23秒前
高分求助中
Pharmacogenomics: Applications to Patient Care, Third Edition 1000
Studien zur Ideengeschichte der Gesetzgebung 1000
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Genera Insectorum: Mantodea, Fam. Mantidæ, Subfam. Hymenopodinæ (Classic Reprint) 800
Ethnicities: Media, Health, and Coping 700
Development of a new synthetic process for the synthesis of (S)-methadone and (S)- and (R)-isomethadone as NMDA receptor antagonists for the treatment of depression 500
A Dissection Guide & Atlas to the Rabbit 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3089386
求助须知:如何正确求助?哪些是违规求助? 2741586
关于积分的说明 7565941
捐赠科研通 2392093
什么是DOI,文献DOI怎么找? 1268497
科研通“疑难数据库(出版商)”最低求助积分说明 614069
版权声明 598692