ROS-responsive probes for low-background optical imaging: a review

Optical imaging is a facile tool for visualizing biological processes and disease progression, but its image quality is largely limited by light-induced autofluorescence or background signals. To overcome this issue, low-background optical-imaging techniques including chemiluminescence imaging, afterglow imaging and photoacoustic imaging have been developed, based on their unique working mechanisms, which are: the detection of light emissions from chemical reactions, the cessation of light excitation before signal collection, and the detection of ultrasonic signals instead of light signals, respectively. Stimuli-responsive probes are highly desirable for improved imaging results since they can significantly reduce surrounding interference signals. Reactive oxygen species (ROS), which are closely implicated in a series of diseases such as cancer and inflammation, are frequently employed as initiators for responsive agents to selectively change the imaging signal. Thus, ROS-responsive agents incorporated into low-background imaging techniques can achieve a more promising imaging quality. In this review, recent advances in ROS-responsive probes for low-background optical-imaging techniques are summarized. Moreover, the approaches to improving the sensitivity of probes and tissue penetration depth are discussed in detail. In particular, we highlight the reaction mechanisms between the probes and ROS, revealing the potential for low-background optical imaging.