参考文献
基因组
人病毒体
基因组
生物
病毒分类
计算生物学
分类学(生物学)
细菌分类学
基因
遗传学
生态学
16S核糖体RNA
作者
Ho Bin Jang,Benjamin Bolduc,Olivier Zablocki,Jens H. Kuhn,Simon Roux,Evelien M. Adriaenssens,J. Rodney Brister,Andrew M. Kropinski,Mart Krupovìč,Rob Lavigne,Dann Turner,Matthew B. Sullivan
标识
DOI:10.1038/s41587-019-0100-8
摘要
Microbiomes from every environment contain a myriad of uncultivated archaeal and bacterial viruses, but studying these viruses is hampered by the lack of a universal, scalable taxonomic framework. We present vConTACT v.2.0, a network-based application utilizing whole genome gene-sharing profiles for virus taxonomy that integrates distance-based hierarchical clustering and confidence scores for all taxonomic predictions. We report near-identical (96%) replication of existing genus-level viral taxonomy assignments from the International Committee on Taxonomy of Viruses for National Center for Biotechnology Information virus RefSeq. Application of vConTACT v.2.0 to 1,364 previously unclassified viruses deposited in virus RefSeq as reference genomes produced automatic, high-confidence genus assignments for 820 of the 1,364. We applied vConTACT v.2.0 to analyze 15,280 Global Ocean Virome genome fragments and were able to provide taxonomic assignments for 31% of these data, which shows that our algorithm is scalable to very large metagenomic datasets. Our taxonomy tool can be automated and applied to metagenomes from any environment for virus classification. Classification of archaeal and bacterial viruses can be automated with an algorithm that identifies relationships on the basis of shared gene content.
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