氧自由基吸收能力
化学
多糖
阿布茨
岩藻糖
半乳糖
单糖
抗氧化剂
褐藻糖胶
特罗洛克
色谱法
甘露糖
葡萄糖醛酸
海带
食品科学
DPPH
生物化学
植物
生物
藻类
作者
Chun Cui,Jianghong Lu,Dongxiao Sun‐Waterhouse,Lixia Mu,Weizheng Sun,Mouming Zhao,Haifeng Zhao
标识
DOI:10.1016/j.lwt.2016.07.005
摘要
In the present study, polysaccharides from Laminaria japonica (LJPA-P) were investigated for their structural characteristics and antioxidant activity. Three acidic polysaccharides LJPA-P1, LJPA-P2 and LJPA-P3 were obtained from Laminaria japonica by sequential hot water and citric acid extraction, and fractionation by DEAE-Sepharose fast flow chromatography and serial NaCl solution elutions. Results showed that three acidic polysaccharides differed in their monosaccharide compositions, sulfation, glycosidic linkages, molecular weights and antioxidant activities. LJPA-P3 was unique in its high sulfate group and galactose contents, molar ratio of galactose, mannose and fucose (26.1: 1.3: 1) and dominance of 1,3- and 1-linked galactose, exhibiting remarkably high oxygen radical absorbance capacity (ORAC, 1247.22 μmol Trolox/g) and ABTS radical scavenging activity (up to 70%), which was quite different from those of LJPA-P1 and LJPA-P2. Therefore, brown seaweed Laminaria japonica represents a good source of polysaccharides with significant antioxidant activity.
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