Stress response and signalling of a low-temperature bioaugmentation system in decentralized wastewater treatment: Degradation characteristics, community structure, and bioaugmented mechanisms

Low temperatures present challenges for stable wastewater treatment operations in cold regions. Low-temperature effective microorganisms (LTEM) were added as a bioaugmentation strategy at a decentralized treatment facility to improve performance. The effects of a low-temperature bioaugmentation system (LTBS) with LTEM at low temperatures (4 °C) on organic pollutant performance, microbial community changes, and the metabolic pathways of functional genes and functional enzymes were studied. To explore the bioaugmentation mechanism of LTBS based on stress response and signalling. The results showed that the start-up time of the LTBS (S2) with LTEM was shorter (8 days) and that it removed COD and NH4+-N at higher rates (87 % and 72 %, respectively) at 4 °C. LTEM effectively degraded complex macromolecular organics into small molecular organics, and decomposing sludge flocs and the changing the extracellular polymeric substances (EPS) structure removed more organics and nitrogen. LTEM and local microbial communities (nitrifying and denitrifying bacteria) improved the ability of organic matter degradation and denitrification of the LTBS and formed a core microbial community dominated by LTEM (Bacillus and Pseudomonas). Finally, based on the functional enzymes and metabolic pathways of the LTBS, a low-temperature strengthening mechanism consisting of 6 cold stress responses and signal pathways under low temperatures was formed. This study demonstrated that the LTEM-dominated LTBS could provide an engineering alternative for future decentralized wastewater treatment in cold regions.