亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Inhibition of NF-kB/IL-6/JAK2/STAT3 Pathway and Epithelial-Mesenchymal Transition in Breast Cancer Cells by Azilsartan

上皮-间质转换 癌症研究 细胞凋亡 细胞周期 细胞生长 转移 活力测定 癌症 化学 乳腺癌 生物 遗传学 生物化学
作者
Rania Alaaeldin,Fares E.M. Ali,Amany Abdlrehim Bekhit,Qing‐Li Zhao,Moustafa Fathy
出处
期刊:Molecules [MDPI AG]
卷期号:27 (22): 7825-7825 被引量:30
标识
DOI:10.3390/molecules27227825
摘要

Metastatic breast cancer is an incurable form of breast cancer that exhibits high levels of epithelial-mesenchymal transition (EMT) markers. Angiotensin II has been linked to various signaling pathways involved in tumor cell growth and metastasis. The aim of this study is to investigate, for the first time, the anti-proliferative activity of azilsartan, an angiotensin II receptor blocker, against breast cancer cell lines MCF-7 and MDA-MB-231 at the molecular level. Cell viability, cell cycle, apoptosis, colony formation, and cell migration assays were performed. RT-PCR and western blotting analysis were used to explain the molecular mechanism. Azilsartan significantly decreased the cancer cells survival, induced apoptosis and cell cycle arrest, and inhibited colony formation and cell migration abilities. Furthermore, azilsartan reduced the mRNA levels of NF-kB, TWIST, SNAIL, SLUG and bcl2, and increased the mRNA level of bax. Additionally, azilsartan inhibited the expression of IL-6, JAK2, STAT3, MMP9 and bcl2 proteins, and increased the expression of bax, c-PARP and cleaved caspase 3 protein. Interestingly, it reduced the in vivo metastatic capacity of MDA-MBA-231 breast cancer cells. In conclusion, the present study revealed, for the first time, the anti-proliferative, apoptotic, anti-migration and EMT inhibition activities of azilsartan against breast cancer cells through modulating NF-kB/IL-6/JAK2/STAT3/MMP9, TWIST/SNAIL/SLUG and apoptosis signaling pathways.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
8秒前
18秒前
玄之又玄完成签到,获得积分10
23秒前
年轻的凝云完成签到 ,获得积分10
24秒前
26秒前
31秒前
32秒前
32秒前
32秒前
Amadeus发布了新的文献求助10
36秒前
38秒前
42秒前
若雨凌风应助Amadeus采纳,获得30
44秒前
大个应助Amadeus采纳,获得10
44秒前
年轻的冰海完成签到,获得积分10
46秒前
52秒前
53秒前
59秒前
1分钟前
1分钟前
1分钟前
t铁核桃1985完成签到 ,获得积分10
1分钟前
1分钟前
1分钟前
1分钟前
1分钟前
奈思完成签到 ,获得积分10
1分钟前
1分钟前
1分钟前
1分钟前
1分钟前
众人皆醉我独醒完成签到,获得积分10
1分钟前
1分钟前
2分钟前
2分钟前
2分钟前
2分钟前
2分钟前
2分钟前
2分钟前
高分求助中
Production Logging: Theoretical and Interpretive Elements 2500
Healthcare Finance: Modern Financial Analysis for Accelerating Biomedical Innovation 2000
Applications of Emerging Nanomaterials and Nanotechnology 1111
Agaricales of New Zealand 1: Pluteaceae - Entolomataceae 1040
Les Mantodea de Guyane Insecta, Polyneoptera 1000
Neuromuscular and Electrodiagnostic Medicine Board Review 700
지식생태학: 생태학, 죽은 지식을 깨우다 600
热门求助领域 (近24小时)
化学 医学 材料科学 生物 工程类 有机化学 生物化学 纳米技术 内科学 物理 化学工程 计算机科学 复合材料 基因 遗传学 物理化学 催化作用 细胞生物学 免疫学 电极
热门帖子
关注 科研通微信公众号,转发送积分 3466835
求助须知:如何正确求助?哪些是违规求助? 3059635
关于积分的说明 9067253
捐赠科研通 2750111
什么是DOI,文献DOI怎么找? 1509039
科研通“疑难数据库(出版商)”最低求助积分说明 697124
邀请新用户注册赠送积分活动 696896