球体
伊立替康
医学
博莱霉素
阿霉素
药物输送
靶向给药
电化学疗法
药理学
碘化丙啶
癌症研究
结直肠癌
药品
癌症
细胞凋亡
化疗
体外
化学
内科学
生物化学
有机化学
程序性细胞死亡
作者
Marie Le Roy,Corentin Alix,Julien Burlaud‐Gaillard,Damien Fouan,William Raoul,Ayache Bouakaz,Emmanuelle Blanchard,Thierry Lecomte,Marie‐Claude Viaud‐Massuard,Noboru Sasaki,Sophie Sérrière,Jean‐Michel Escoffre
标识
DOI:10.1021/acs.molpharmaceut.3c00921
摘要
Tumor spheroids are promising three-dimensional (3D) in vitro tumor models for the evaluation of drug delivery methods. The design of noninvasive and targeted drug methods is required to improve the intratumoral bioavailability of chemotherapeutic drugs and reduce their adverse off-target effects. Among such methods, microbubble-assisted ultrasound (MB-assisted US) is an innovative modality for noninvasive targeted drug delivery. The aim of the present study is to evaluate the efficacy of this US modality for the delivery of bleomycin, doxorubicin, and irinotecan in colorectal cancer (CRC) spheroids. MB-assisted US permeabilized the CRC spheroids to propidium iodide, which was used as a drug model without affecting their growth and viability. Histological analysis and electron microscopy revealed that MB-assisted US affected only the peripheral layer of the CRC spheroids. The acoustically mediated bleomycin delivery induced a significant decrease in CRC spheroid growth in comparison to spheroids treated with bleomycin alone. However, this US modality did not improve the therapeutic efficacy of doxorubicin and irinotecan on CRC spheroids. In conclusion, this study demonstrates that tumor spheroids are a relevant approach to evaluate the efficacy of MB-assisted US for the delivery of chemotherapeutics.
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