In this paper, a twisting sliding mode control (TSMC) method based on nonlinear disturbance observer (NDOB) is proposed for the resistance load disturbance and input voltage change of DC-DC boost converter. First, a nonlinear disturbance observer is designed to estimate the disturbance by using the feedback state information of the system. On this basis, a twisting sliding mode controller is designed to make the output voltage of the system track the given voltage quickly and accurately. Lyapunov stability theorem is used to prove that the designed controller can guarantee the stability of the closed-loop system. Experimental results show that compared with the sliding mode control (SMC) method based on extended state observer (ESO), the proposed control method improves the tracking performance and the robustness to disturbances and uncertainties.