体内
活力测定
胶质瘤
诱导剂
细胞周期
细胞生长
免疫印迹
细胞培养
细胞
化学
生物
癌症研究
细胞生物学
分子生物学
生物化学
基因
生物技术
遗传学
作者
Xin Zhang,Yulian Guo,Hao Li,Lizhang Han
出处
期刊:Journal of Cancer
[Ivyspring International Publisher]
日期:2021-01-01
卷期号:12 (22): 6610-6619
被引量:37
摘要
Objective: To explore the anti-tumor effect of FIN56, a novel ferroptosis inducer, on glioblastoma and its underlying mechanisms.Methods: Two human glioblastoma cell lines, LN229 and U118 were applied in this study.Anti-tumor effect was measured by CCK-8 assay, EdU assay and cell cycle analysis.Fluorescent probes, immunofluorescence, plasmid transfection, shRNA knocking out, reverse transcription PCR, western blot analysis, and transmission electron microscopy were used to study the underlying mechanisms.At last, a subcutaneous nude mice model was used to study the anti-tumor effect of FIN56 in vivo.The GraphPad Prism software program was applied for statistical analysis.Results: FIN56 decreased cell viability, inhibited cell proliferation and caused cell cycle arrest on LN229 and U118 cells.Further study showed that FIN56 induced ferroptosis and induced lysosomal membrane permeabilization in a ferroptosis and transfactor EB dependent manner.Animal study demonstrated that FIN56 inhibited glioma growth and caused ferroptosis in vivo.Conclusion: FIN56 is a promising anti-tumor compound.
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