类有机物
蠕动
结直肠癌
微流控
LGR5型
生物医学工程
细胞生物学
材料科学
生物
纳米技术
癌症
内科学
医学
解剖
作者
Guocheng Fang,Hongxu Lu,Russul Al-Nakashli,Robert Chapman,Yingqi Zhang,Lining Arnold Ju,Gungun Lin,Martina H. Stenzel,Dayong Jin
出处
期刊:Biofabrication
[IOP Publishing]
日期:2021-10-25
卷期号:14 (1): 015006-015006
被引量:44
标识
DOI:10.1088/1758-5090/ac2ef9
摘要
Abstract Peristalsis in the digestive tract is crucial to maintain physiological functions. It remains challenging to mimic the peristaltic microenvironment in gastrointestinal organoid culture. Here, we present a method to model the peristalsis for human colon tumor organoids on a microfluidic chip. The chip contains hundreds of lateral microwells and a surrounding pressure channel. Human colon tumor organoids growing in the microwell were cyclically contracted by pressure channel, mimicking the in vivo mechano-stimulus by intestinal muscles. The chip allows the control of peristalsis amplitude and rhythm and the high throughput culture of organoids simultaneously. By applying 8% amplitude with 8 ∼ 10 times min −1 , we observed the enhanced expression of Lgr5 and Ki67. Moreover, ellipticine-loaded polymeric micelles showed reduced uptake in the organoids under peristalsis and resulted in compromised anti-tumor efficacy. The results indicate the importance of mechanical stimuli mimicking the physiological environment when using in vitro models to evaluate nanoparticles. This work provides a method for attaining more reliable and representative organoids models in nanomedicine.
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