Selenium Status Affects Hypertrophic Growth of Skeletal Muscle in Growing Zebrafish by Mediating Protein Turnover

骨骼肌 斑马鱼 内分泌学 内科学 自噬 心肌细胞 溶酶体 生物 肌萎缩 肌肉肥大 化学 生物化学 基因 细胞凋亡 医学
作者
Li Wang,Jiaojiao Yin,Feng Zhang,Haodong Yu,Feifei Chen,Ziyi Zhang,Xuezhen Zhang
出处
期刊:Journal of Nutrition [Elsevier BV]
卷期号:151 (7): 1791-1801 被引量:10
标识
DOI:10.1093/jn/nxab082
摘要

Selenium (Se) status is closely related to skeletal muscle physiological status. However, its influence on skeletal muscle growth has not been well studied.This study aimed to analyze the impacts of overall Se status (deficient, adequate, and high) on skeletal muscle growth using a growing zebrafish model.Zebrafish (1.5-mo-old) were fed graded levels of Se (deficient: 0.10 mg Se/kg; marginally deficient: 0.22 mg Se/kg; adequate: 0.34 mg Se/kg; high: 0.44, 0.57, and 0.69 mg Se/kg) as Se-enriched yeast for 30 d. Zebrafish growth, and Se accumulation, selenoenzyme activity, selenotranscriptome profiles, and oxidative status in the whole body, and selenotranscriptome profiles, histological characteristics, biochemicals, and gene and protein expression profiles related to muscle growth in the skeletal muscle were analyzed by model fitting and/or 1-factor ANOVA.Se status biomarkers within the whole body and skeletal muscle indicated that 0.34 mg Se/kg was adequate for growing zebrafish. For biomarkers related to skeletal muscle growth, compared with 0.34 mg Se/kg, 0.10 mg Se/kg decreased the white muscle cross-sectional area (WMCSA) and the mean diameter of white muscle fibers (MDWMF) by 14.4%-15.1%, inhibited protein kinase B-target of rapamycin complex 1 signaling by 63.7%-68.5%, and stimulated the autophagy-lysosome pathway by 1.07 times and the ubiquitin-proteasome pathway (UPP) by 96.0% (P < 0.05), whereas 0.22 mg Se/kg only decreased the WMCSA by 7.8% (P < 0.05); furthermore, 0.44 mg Se/kg had no clear effects on skeletal muscle biomarkers, whereas 0.57-0.69 mg Se/kg decreased the WMCSA and MDWMF by 6.3%-25.9% and 5.1%-21.3%, respectively, and stimulated the UPP by 2.23 times (P < 0.05).A level of 0.34 mg Se/kg is adequate for the growth of zebrafish skeletal muscle, whereas ≤0.10 and ≥0.57 mg Se/kg are too low or too high, respectively, for maintaining efficient protein accretion and normal hypertrophic growth.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
wit完成签到,获得积分10
1秒前
2秒前
圈儿完成签到,获得积分10
2秒前
柯尔丝完成签到,获得积分10
3秒前
3秒前
5秒前
英姑应助千纸鹤采纳,获得10
5秒前
ZQ发布了新的文献求助10
5秒前
6秒前
安安发布了新的文献求助10
6秒前
8秒前
盼盼发布了新的文献求助10
10秒前
ZXFFF发布了新的文献求助10
10秒前
wendy发布了新的文献求助10
11秒前
11秒前
xu关闭了xu文献求助
12秒前
yiw完成签到,获得积分10
13秒前
可靠笑翠发布了新的文献求助10
13秒前
13秒前
14秒前
17秒前
17秒前
inin发布了新的文献求助20
19秒前
科研通AI2S应助琪琪的采纳,获得10
19秒前
卓隶发布了新的文献求助10
19秒前
SciGPT应助董海涛采纳,获得10
20秒前
傻傻的小虾米完成签到,获得积分10
20秒前
20秒前
21秒前
科研通AI2S应助科研通管家采纳,获得10
21秒前
思源应助清新的访冬采纳,获得10
21秒前
ding应助科研通管家采纳,获得10
21秒前
21秒前
wulin应助科研通管家采纳,获得10
22秒前
华仔应助山猪吃细糠采纳,获得10
22秒前
zho应助科研通管家采纳,获得10
22秒前
研友_VZG7GZ应助科研通管家采纳,获得10
22秒前
烟花应助科研通管家采纳,获得10
22秒前
我是老大应助科研通管家采纳,获得10
22秒前
科研通AI5应助科研通管家采纳,获得30
22秒前
高分求助中
Picture Books with Same-sex Parented Families: Unintentional Censorship 1000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Microbiology and Health Benefits of Traditional Alcoholic Beverages 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 310
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3979984
求助须知:如何正确求助?哪些是违规求助? 3524121
关于积分的说明 11219921
捐赠科研通 3261562
什么是DOI,文献DOI怎么找? 1800703
邀请新用户注册赠送积分活动 879263
科研通“疑难数据库(出版商)”最低求助积分说明 807232