Effects of the molluscicide candidate PPU06 on alkaline phosphatase in the golden apple snails determined using a near-infrared fluorescent probe

福寿螺 杀螺剂 荧光 碱性磷酸酶 蜗牛 化学 生物 解剖 生物化学 生态学 量子力学 物理
作者
Changxiaoxi Liu,Yang Suo,Yimu Qiao,Yu-Qiang Zhao,Weisi Wang,Mingxuan Jia,Yanqi He,Ying Zhou,Liping Duan
出处
期刊:Chinese Chemical Letters [Elsevier]
卷期号:32 (5): 1809-1813 被引量:12
标识
DOI:10.1016/j.cclet.2020.12.029
摘要

We constructed a reaction-based near-infrared fluorescent probe (Niap) to specifically identify alkaline phosphatase (ALP) with fast red fluorescence enhancement. Based on the positive concentration-dependent manner between the fluorescent intensity of the Niap and ALP, probe Niap was used to study the ALP enrichment and variation in golden apple snails (Pomacea canaliculata) exposed to the molluscicide candidate PPU06. After treatment with different concentrations of PPU06 over various times, three organs of the surviving snails, liver, stomach and plantaris, were frozen and sectioned for fluorescent imaging experiments. With increased PPU06 concentration, red fluorescence substantially increased in the liver and reached a maximum within 24 h when the PPU06 concentration was 0.75 mg/L. No obvious changes in the stomach or foot plantaris were found. It showed PPU06 caused liver injury and stimulated the increase of ALP in the liver of P. canaliculata. This study demonstrates a rapid ALP fluorescent identification method that can be used to study the effects of PPU06 on P. canaliculata. It also provides optical evidence that may aid in the discovery of new chemistry for snail control.
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